Imperial College London
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DrLorenzoDi Michele

Faculty of Natural Sciences, Department of Chemistry

Honorary Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 3262l.di-michele Website

 
 
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Location

 

Molecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Clowsley:2021:10.1038/s41467-020-20686-z,
author = {Clowsley, AH and Kaufhold, WT and Lutz, T and Meletiou, A and Di, Michele L and Soeller, C},
doi = {10.1038/s41467-020-20686-z},
journal = {Nature Communications},
pages = {1--10},
title = {Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy},
url = {http://dx.doi.org/10.1038/s41467-020-20686-z},
volume = {12},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA ‘imagers’ to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.
AU  - Clowsley,AH
AU  - Kaufhold,WT
AU  - Lutz,T
AU  - Meletiou,A
AU  - Di,Michele L
AU  - Soeller,C
DO  - 10.1038/s41467-020-20686-z
EP  - 10
PY  - 2021///
SN  - 2041-1723
SP  - 1
TI  - Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-020-20686-z
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000613038200011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/s41467-020-20686-z
UR  - http://hdl.handle.net/10044/1/93390
VL  - 12
ER  -
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