Imperial College London
Alternate

Professor Nilay Shah OBE FREng

Faculty of Engineering, Department of Chemical Engineering

Professor of Process Systems Engineering
 
 
 
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Contact

 

+44 (0)20 7594 6621n.shah

 
 
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Assistant

 

Miss Jessica Baldock +44 (0)20 7594 5699

 
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Location

 

ACEX 522ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Klymenko:2016:10.1002/aic.15388,
author = {Klymenko, O and Royle, K and Polizzi, KM and Shah, N and Kontoravdi, C},
doi = {10.1002/aic.15388},
journal = {AICHE Journal},
pages = {2959--2973},
title = {Designing an Artificial Golgi Reactor to achieve targeted glycosylation of monoclonal antibodies},
url = {http://dx.doi.org/10.1002/aic.15388},
volume = {62},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The therapeutic efficacy of monoclonal antibodies (mAbs) is dependent upon their glycosylationpatterns. As the largest group of currently approved biopharmaceuticals, the microheterogeneity inmAb oligosaccharide profiles deriving from mammalian cell production is a challenge to thebiopharmaceutical industry. Disengaging the glycosylation process from the cell may offer significantenhancement of product quality and allow better control and reproducibility in line with the Quality byDesign paradigm. Three potential designs of an Artificial Golgi reactor implementing targeted sequentialglycosylation of mAbs are proposed including a (i) microcapillary film reactor, (ii) packed bed reactorwith non-porous pellets, and (iii) packed bed reactor with porous pellets. Detailed mathematical modelsare developed to predict their performance for a range of design and operational parameters. While allthree reactor designs can achieve desired conversion levels, the choice of a particular one depends onthe required throughput and the associated cost of enzymes and co-substrates.
AU  - Klymenko,O
AU  - Royle,K
AU  - Polizzi,KM
AU  - Shah,N
AU  - Kontoravdi,C
DO  - 10.1002/aic.15388
EP  - 2973
PY  - 2016///
SN  - 0001-1541
SP  - 2959
TI  - Designing an Artificial Golgi Reactor to achieve targeted glycosylation of monoclonal antibodies
T2  - AICHE Journal
UR  - http://dx.doi.org/10.1002/aic.15388
UR  - http://hdl.handle.net/10044/1/34029
VL  - 62
ER  -
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