Imperial College London
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ProfessorPaulFreemont

Faculty of Medicine, Department of Infectious Disease

Chair in Protein Crystallography
 
 
 
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Contact

 

+44 (0)20 7594 5327p.freemont

 
 
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Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wang:2004:10.1083/jcb.200305142,
author = {Wang, J and Shiels, C and Sasieni, P and Wu, PJ and Islam, SA and Freemont, PS and Sheer, D},
doi = {10.1083/jcb.200305142},
journal = {Journal of Cell Biology},
pages = {515--526},
title = {Promyelocytic leukemia nuclear bodies associate with transcriptionally active genomic regions},
url = {http://dx.doi.org/10.1083/jcb.200305142},
volume = {164},
year = {2004}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The promyelocytic leukemia (PML) protein is aggregated into nuclear bodies that are associated with diverse nuclear processes. Here, we report that the distance between a locus and its nearest PML body correlates with the transcriptional activity and gene density around the locus. Genes on the active X chromosome are more significantly associated with PML bodies than their silenced homologues on the inactive X chromosome. We also found that a histone-encoding gene cluster, which is transcribed only in S-phase, is more strongly associated with PML bodies in S-phase than in G0/G1 phase of the cell cycle. However, visualization of specific RNA transcripts for several genes showed that PML bodies were not themselves sites of transcription for these genes. Furthermore, knock-down of PML bodies by RNA interference did not preferentially change the expression of genes closely associated with PML bodies. We propose that PML bodies form in nuclear compartments of high transcriptional activity, but they do not directly regulate transcription of genes in these compartments.
AU  - Wang,J
AU  - Shiels,C
AU  - Sasieni,P
AU  - Wu,PJ
AU  - Islam,SA
AU  - Freemont,PS
AU  - Sheer,D
DO  - 10.1083/jcb.200305142
EP  - 526
PY  - 2004///
SN  - 0021-9525
SP  - 515
TI  - Promyelocytic leukemia nuclear bodies associate with transcriptionally active genomic regions
T2  - Journal of Cell Biology
UR  - http://dx.doi.org/10.1083/jcb.200305142
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000189077200018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/69626
VL  - 164
ER  -
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