Imperial College London
Alternate

ProfessorPietroSpanu

Faculty of Natural Sciences, Department of Life Sciences

Professor of Molecular Plant Pathology
 
 
 
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Contact

 

+44 (0)20 7594 5384p.spanu Website

 
 
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Location

 

610Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pennington:2016:10.1021/acs.jproteome.5b00732,
author = {Pennington, HG and Gheorghe, DM and Damerum, A and Pliego, C and Spanu, PD and Cramer, R and Bindschedler, LV},
doi = {10.1021/acs.jproteome.5b00732},
journal = {Journal of Proteome Research},
pages = {826--839},
title = {Interactions between the Powdery Mildew Effector BEC1054 and Barley Proteins Identify Candidate Host Targets},
url = {http://dx.doi.org/10.1021/acs.jproteome.5b00732},
volume = {15},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - There are over 500 candidate secreted effector proteins (CSEPs) or Blumeria effector candidates (BECs) specific to the barley powdery mildew pathogen Blumeria graminis f.sp. hordei. The CSEP/BEC proteins are expressed and predicted to be secreted by biotrophic feeding structures called haustoria. Eight BECs are required for the formation of functional haustoria. These include the RNase-like effector BEC1054 (synonym CSEP0064). In order to identify host proteins targeted by BEC1054, recombinant BEC1054 was expressed in E. coli, solubilized, and used in pull-down assays from barley protein extracts. Many putative interactors were identified by LC-MS/MS after subtraction of unspecific binders in negative controls. Therefore, a directed yeast-2-hybrid assay, developed to measure the effectiveness of the interactions in yeast, was used to validate putative interactors. We conclude that BEC1054 may target several host proteins, including a glutathione-S-transferase, a malate dehydrogenase, and a pathogen-related-5 protein isoform, indicating a possible role for BEC1054 in compromising well-known key players of defense and response to pathogens. In addition, BEC1054 interacts with an elongation factor 1 gamma. This study already suggests that BEC1054 plays a central role in barley powdery mildew virulence by acting at several levels.
AU  - Pennington,HG
AU  - Gheorghe,DM
AU  - Damerum,A
AU  - Pliego,C
AU  - Spanu,PD
AU  - Cramer,R
AU  - Bindschedler,LV
DO  - 10.1021/acs.jproteome.5b00732
EP  - 839
PY  - 2016///
SN  - 1535-3907
SP  - 826
TI  - Interactions between the Powdery Mildew Effector BEC1054 and Barley Proteins Identify Candidate Host Targets
T2  - Journal of Proteome Research
UR  - http://dx.doi.org/10.1021/acs.jproteome.5b00732
UR  - http://hdl.handle.net/10044/1/40653
VL  - 15
ER  -
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