Imperial College London
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Dr Richard J. Gill

Faculty of Natural Sciences, Department of Life Sciences (Silwood Park)

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2215r.gill Website

 
 
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Location

 

N2.13MunroSilwood Park

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Summary

 

Publications

Citation

BibTex format

@article{Rother:2021:10.1007/s00441-021-03482-z,
author = {Rother, L and Kraft, N and Smith, DB and el, Jundi B and Gill, RJ and Pfeiffer, K},
doi = {10.1007/s00441-021-03482-z},
journal = {Cell and Tissue Research},
pages = {29--45},
title = {A micro-CT-based standard brain atlas of the bumblebee},
url = {http://dx.doi.org/10.1007/s00441-021-03482-z},
volume = {386},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>In recent years, bumblebees have become a prominent insect model organism for a variety of biological disciplines, particularly to investigate learning behaviors as well as visual performance. Understanding these behaviors and their underlying neurobiological principles requires a clear understanding of brain anatomy. Furthermore, to be able to compare neuronal branching patterns across individuals, a common framework is required, which has led to the development of 3D standard brain atlases in most of the neurobiological insect model species. Yet, no bumblebee 3D standard brain atlas has been generated. Here we present a brain atlas for the buff-tailed bumblebee <jats:italic>Bombus terrestris</jats:italic> using micro-computed tomography (micro-CT) scans as a source for the raw data sets, rather than traditional confocal microscopy, to produce the first ever micro-CT-based insect brain atlas. We illustrate the advantages of the micro-CT technique, namely, identical native resolution in the three cardinal planes and 3D structure being better preserved. Our <jats:italic>Bombus terrestris</jats:italic> brain atlas consists of 30 neuropils reconstructed from ten individual worker bees, with micro-CT allowing us to segment neuropils completely intact, including the lamina, which is a tissue structure often damaged when dissecting for immunolabeling. Our brain atlas can serve as a platform to facilitate future neuroscience studies in bumblebees and illustrates the advantages of micro-CT for specific applications in insect neuroanatomy.</jats:p>
AU  - Rother,L
AU  - Kraft,N
AU  - Smith,DB
AU  - el,Jundi B
AU  - Gill,RJ
AU  - Pfeiffer,K
DO  - 10.1007/s00441-021-03482-z
EP  - 45
PY  - 2021///
SN  - 0302-766X
SP  - 29
TI  - A micro-CT-based standard brain atlas of the bumblebee
T2  - Cell and Tissue Research
UR  - http://dx.doi.org/10.1007/s00441-021-03482-z
UR  - http://hdl.handle.net/10044/1/90270
VL  - 386
ER  -
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