Publications
464 results found
Rau T, Nose M, Remmers U, et al., 2003, Overexpression of wild-type Gαi-2 suppresses β-adrenergic signaling in cardiac myocytes, FASEB JOURNAL, Vol: 17, Pages: 523-+, ISSN: 0892-6638
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- Citations: 35
El-Armouche A, Rau T, Zolk O, et al., 2003, Evidence for protein phosphatase inhibitor-1 playing an amplifier role in β-adrenergic signaling in cardiac myocytes, FASEB JOURNAL, Vol: 17, Pages: 437-+, ISSN: 0892-6638
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- Citations: 94
Chaudhri B, Del Monte F, Hajjar RJ, et al., 2002, Interaction between increased SERCA2a activity and β-adrenoceptor stimulation in adult rabbit myocytes, American Journal of Physiology - Heart and Circulatory Physiology, Vol: 283, ISSN: 0363-6135
Sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA)2a overexpression and phospholamban depletion have been shown to have beneficial effects on contractility in heart failure. However, the high sympathetic tone during development of failure may interact with increases in SERCA2a activity in potentially deleterious ways. We used adenoviral vectors to overexpress SERCA2a or partially downregulate phospholamban in adult rabbit ventricular myocytes in culture and studied the responses of these cells to β-adrenoceptor stimulation. SERCA2a overexpression and phospholamban depletion had quantitatively similar effects on basal contraction amplitude and in accelerating relaxation. Increasing SERCA2a activity by either strategy had little effect on the increase in contraction amplitude or incidence of arrhythmias with increasing isoproterenol. Maximum acceleration of relaxation by β-adrenoceptor stimulation was similar to that produced by SERCA2a overexpression. Isoproterenol treatment of SERCA2a-overexpressing or phospholamban-deficient myocytes produced a further modest decrease in relaxation time, with similar final values in both groups. We find no evidence for Ca2+ overload induced by SERCA2a overexpression alone or in combination with catecholamines.
Lewis CJ, Gong H, Koch WJ, et al., 2002, Adenovirally-overexpressed beta 2-adrenoceptors enhance the contractile response to CGP 12177A in adult rat cardiomyocytes, Meeting of the British-Pharmacological-Society, Publisher: NATURE PUBLISHING GROUP, ISSN: 0007-1188
Chaudhri B, Del Monte F, Hajjar RJ, et al., 2002, Interaction between increased SERCA2a activity and β-adrenoceptor stimulation in adult rabbit myocytes, AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, Vol: 283, Pages: H2450-H2457, ISSN: 0363-6135
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- Citations: 13
Harding SE, Del Monte F, 2002, Dissociation of hypertrophic growth from changes in myocyte contractile function, JOURNAL OF CARDIAC FAILURE, Vol: 8, Pages: S415-S420, ISSN: 1071-9164
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- Citations: 1
Gorelik J, Gu YC, Spohr HA, et al., 2002, Ion channels in small cells and subcellular structures can be studied with a smart patch-clamp system, BIOPHYSICAL JOURNAL, Vol: 83, Pages: 3296-3303, ISSN: 0006-3495
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- Citations: 92
Pathan N, Sandiford C, Harding SE, et al., 2002, Characterization of a myocardial depressant factor in meningococcal septicemia, CRITICAL CARE MEDICINE, Vol: 30, Pages: 2191-2198, ISSN: 0090-3493
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- Citations: 27
Gorelik J, Harding SE, Shevchuk AI, et al., 2002, Taurocholate induces changes in rat cardiomyocyte contraction and calcium dynamics, CLINICAL SCIENCE, Vol: 103, Pages: 191-200, ISSN: 0143-5221
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- Citations: 74
Terracciano CMN, Hajjar RJ, Harding SE, 2002, Overexpression of SERCA2a accelerates repolarisation in rabbit ventricular myocytes, CELL CALCIUM, Vol: 31, Pages: 299-305, ISSN: 0143-4160
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- Citations: 19
Gong HB, Sun H, Koch WJ, et al., 2002, Specific β<sub>2</sub>AR blocker ICI 118,551 actively decreases contraction through a G<sub>i</sub>-coupled form of the β<sub>2</sub>AR in myocytes from failing human heart, CIRCULATION, Vol: 105, Pages: 2497-2503, ISSN: 0009-7322
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- Citations: 88
Ranu HK, Terracciano CMN, Davia K, et al., 2002, Effects of Na<SUP>+</SUP>/Ca<SUP>2+</SUP>-exchanger overexpression on excitation-contraction coupling in adult rabbit ventricular myocytes, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 34, Pages: 389-400, ISSN: 0022-2828
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- Citations: 46
Gu YC, Gorelik J, Spohr HA, et al., 2002, High-resolution scanning patch-clamp: new insights into cell function, FASEB JOURNAL, Vol: 16, Pages: 748-+, ISSN: 0892-6638
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- Citations: 65
del Monte F, Harding SE, Dec GW, et al., 2002, Targeting phospholamban by gene transfer in human heart failure, CIRCULATION, Vol: 105, Pages: 904-907, ISSN: 0009-7322
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- Citations: 187
Song GJ, Harding SE, Duchen MR, et al., 2002, Altered mechanical properties and intracellular calcium signaling in cardiomyocytes from annexin 6 null-mutant mice, FASEB JOURNAL, Vol: 16, Pages: 622-+, ISSN: 0892-6638
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- Citations: 41
Song G, Harding SE, Duchen MR, et al., 2002, Altered mechanical properties and intracellular calcium signaling in cardiomyocytes from annexin 6 null-mutant mice., The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol: 16, Pages: 622-624
Annexin 6 is one of a widely expressed family of calcium-binding proteins found in most mammalian tissues, including the heart. Several studies have implicated annexin 6 in the regulation of intracellular Ca2+ signaling, and it has been shown in vitro to act as a modulator of the sarcoplasmic reticulum Ca2+-release channel, cardiac L-type calcium channel, and Na+/Ca2+ exchanger. To investigate the role of annexin 6 in intact cardiomyocytes, we used mice containing a targeted disruption of the annexin 6 gene. Compared with controls, the myocytes of annexin 6 null-mutant mice demonstrated a significant increase in the rates of shortening and relengthening. Intracellular Ca2+ transients in fura-2-loaded cardiomyocytes induced by caffeine showed a normal baseline and amplitude, whereas the rate of decay was doubled in annexin 6-/- myocytes compared with control mice. These results show that annexin 6 knockout in the mouse leads to an increase in myocyte contractility and faster diastolic Ca2+ removal from the cytoplasm. In light of published findings showing annexin 6 to be down-regulated in end-stage heart failure, these results are consistent with a role for annexin 6 as a negative inotropic factor in the regulation of cardiomyocyte mechanics.
Chaudhri B, Hajjar RJ, Harding SE, et al., 2001, Effects of over-expression of SERCA2a on action potential duration, Ca<SUP>2+</SUP> entry and sarcoplasmic reticulum Ca<SUP>2+</SUP> content in rabbit ventricular myocytes, Scientific Meeting of the Physiological-Society, Publisher: WILEY, Pages: 74P-74P, ISSN: 0022-3751
Sarkar D, Vallance P, Harding SE, 2001, Nitric oxide: not just a negative inotrope, EUROPEAN JOURNAL OF HEART FAILURE, Vol: 3, Pages: 527-534, ISSN: 1388-9842
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- Citations: 14
Watt DJ, Goldring K, Harding SE, et al., 2001, A preliminary study on the effect of galectin-1 on cardiomyocytes, NEUROMUSCULAR DISORDERS, Vol: 11, Pages: 651-651, ISSN: 0960-8966
Shevchuk AI, Gorelik J, Harding SE, et al., 2001, Simultaneous measurement of Ca<SUP>2+</SUP> and cellular dynamics:: Combined scanning ion conductance and optical microscopy to study contracting cardiac myocytes, BIOPHYSICAL JOURNAL, Vol: 81, Pages: 1759-1764, ISSN: 0006-3495
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- Citations: 139
del Monte F, Hajjar RJ, Harding SE, 2001, Overwhelming evidence of the beneficial effects of SERCA gene transfer in heart failure, CIRCULATION RESEARCH, Vol: 88, Pages: E66-E66, ISSN: 0009-7330
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- Citations: 50
Davia K, Bernobich E, Ranu HK, et al., 2001, SERCA2a overexpression decreases the incidence of aftercontractions in adult rabbit ventricular myocytes, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 33, Pages: 1005-1015, ISSN: 0022-2828
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- Citations: 68
Heubach JF, Graf EM, Molenaar P, et al., 2001, Murine ventricular L-type Ca<SUP>2+</SUP> current is enhanced by zinterol <i>via</i> β<sub>1</sub>-adrenoceptors, and is reduced in TG4 mice overexpressing the human β<sub>2</sub>-adrenoceptor, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 133, Pages: 73-82, ISSN: 0007-1188
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- Citations: 20
Sarkar D, Vallance P, Amirmansour C, et al., 2000, Positive inotropic effects of NO donors in isolated guinea-pig and human cardiomyocytes independent of NO species and cyclic nucleotides, CARDIOVASCULAR RESEARCH, Vol: 48, Pages: 430-439, ISSN: 0008-6363
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- Citations: 26
Vaughn DW, 2000, Invited commentary: Dengue lessons from Cuba, AMERICAN JOURNAL OF EPIDEMIOLOGY, Vol: 152, Pages: 800-803, ISSN: 0002-9262
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- Citations: 55
Gong HB, Adamson DL, Ranu HK, et al., 2000, The effect of Gi-protein inactivation on basal, and β<sub>1</sub>- and β<sub>2</sub>AR-stimulated contraction of myocytes from transgenic mice overexpressing the β<sub>2</sub>-adrenoceptor, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 131, Pages: 594-600, ISSN: 0007-1188
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- Citations: 43
Yokoyama H, Gunasegaram S, Harding SE, et al., 2000, Sarcolemmal Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger activity and expression in human ventricular myocardium, JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, Vol: 36, Pages: 534-+, ISSN: 0735-1097
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- Citations: 104
Adamson DL, Money-Kyrle ARW, Harding SE, 2000, Functional evidence for a cyclic-AMP related mechanism of action of the β<sub>2</sub>-adrenoceptor in human ventricular myocytes, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 32, Pages: 1353-1360, ISSN: 0022-2828
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- Citations: 12
Ranu HK, Mak JCW, Barnes PJ, et al., 2000, G<sub>i</sub>-dependent suppression of β<sub>1</sub>-adrenoceptor effects in ventricular myocytes from NE-treated guinea pigs, AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, Vol: 278, Pages: H1807-H1814, ISSN: 0363-6135
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- Citations: 15
Adamson DL, Harding SE, 2000, Rp-cAMPS has no effect on adenosine A<sub>1</sub> receptors in guinea-pig cardiomyocytes, BASIC RESEARCH IN CARDIOLOGY, Vol: 95, Pages: 114-118, ISSN: 0300-8428
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- Citations: 1
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