Imperial College London
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ProfessorAnneDell

Faculty of Natural Sciences, Department of Life Sciences

Professor of Carbohydrate Bichemistry
 
 
 
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Contact

 

a.dell

 
 
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Location

 

101BSir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@inbook{Haslam:2015:10.1007/978-4-431-54841-6_87,
author = {Haslam, SM and Pang, PC and Antonopoulos, A and Dell, A},
booktitle = {Glycoscience: Biology and Medicine},
doi = {10.1007/978-4-431-54841-6_87},
pages = {69--77},
title = {Mass spectrometric analyses of cell and tissue glycomes},
url = {http://dx.doi.org/10.1007/978-4-431-54841-6_87},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - CHAP
AB  - This chapter discusses mass spectrometric (MS) strategies for mammalian cell and tissue glycomics and places the principles underlying them within a historical framework. Ultrahigh-sensitivity MALDI-TOF/TOF glycomic methodologies are based on the analysis of permethylated derivatives of pools of glycans that are released from glycoproteins or glycolipids enzymatically or chemically. Very complex mixtures from biological extracts of cells and tissues can be screened in this way, thereby revealing the types of glycans present and, importantly, providing clues to structures that are likely to be functionally important. Optimal glycomic workflows are illustrated by glycomic data from human cytolytic T lymphocytes. Fragmentation pathways that are central to glycomics are briefly outlined, and the use of the Glyco Work Bench tool to assist fragment ion annotation is briefly explained. Finally, the open-access data repositories of the Consortium for Functional Glycomics, which contain glycomics data for murine and human hematopoietic cell populations, are described.
AU  - Haslam,SM
AU  - Pang,PC
AU  - Antonopoulos,A
AU  - Dell,A
DO  - 10.1007/978-4-431-54841-6_87
EP  - 77
PY  - 2015///
SN  - 9784431548409
SP  - 69
TI  - Mass spectrometric analyses of cell and tissue glycomes
T1  - Glycoscience: Biology and Medicine
UR  - http://dx.doi.org/10.1007/978-4-431-54841-6_87
ER  -
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