Imperial College London
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ProfessorAnneDell

Faculty of Natural Sciences, Department of Life Sciences

Professor of Carbohydrate Bichemistry
 
 
 
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Contact

 

a.dell

 
 
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Location

 

101BSir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mkhikian:2016:10.7554/eLife.14314,
author = {Mkhikian, H and Mortales, C-L and Zhou, RW and Khachikyan, K and Wu, G and Haslam, SM and Kavarian, P and Dell, A and Demetriou, M},
doi = {10.7554/eLife.14314},
journal = {eLife},
pages = {1--27},
title = {Golgi self-correction generates bioequivalent glycans to preserve cellular homeostasis},
url = {http://dx.doi.org/10.7554/eLife.14314},
volume = {5},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Essential biological systems employ self-correcting mechanisms to maintain cellular homeostasis. Mammalian cell function is dynamically regulated by the interaction of cell surface galectins with branched N-glycans. Here we report that N-glycan branching deficiency triggers the Golgi to generate bioequivalent N-glycans that preserve galectin-glycoprotein interactions and cellular homeostasis. Galectins bind N-acetyllactosamine (LacNAc) units within N-glycans initiated from UDP-GlcNAc by the medial-Golgi branching enzymes as well as the trans-Golgi poly-LacNAc extension enzyme β1,3-N-acetylglucosaminyltransferase (B3GNT). Marginally reducing LacNAc content by limiting N-glycans to three branches results in T-cell hyperactivity and autoimmunity; yet further restricting branching does not produce a more hyperactive state. Rather, new poly-LacNAc extension by B3GNT maintains galectin binding and immune homeostasis. Poly-LacNAc extension is triggered by redistribution of unused UDP-GlcNAc from the medial to trans-Golgi via inter-cisternal tubules. These data demonstrate the functional equivalency of structurally dissimilar N-glycans and suggest a self-correcting feature of the Golgi that sustains cellular homeostasis.
AU  - Mkhikian,H
AU  - Mortales,C-L
AU  - Zhou,RW
AU  - Khachikyan,K
AU  - Wu,G
AU  - Haslam,SM
AU  - Kavarian,P
AU  - Dell,A
AU  - Demetriou,M
DO  - 10.7554/eLife.14314
EP  - 27
PY  - 2016///
SN  - 2050-084X
SP  - 1
TI  - Golgi self-correction generates bioequivalent glycans to preserve cellular homeostasis
T2  - eLife
UR  - http://dx.doi.org/10.7554/eLife.14314
UR  - https://elifesciences.org/articles/14814
UR  - http://hdl.handle.net/10044/1/33848
VL  - 5
ER  -
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