Imperial College London

Professor DameMandyFisher

Faculty of MedicineInstitute of Clinical Sciences

Head of the Institute of Clinical Sciences
 
 
 
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Contact

 

+44 (0)20 3313 8236amanda.fisher

 
 
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Assistant

 

Ms Alessandra Lisini +44 (0)20 3313 8236

 
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Location

 

CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

119 results found

Cantone I, Dharmalingam G, Chan Y-W, Kohler A-C, Lenhard B, Merkenschlager M, Fisher AGet al., 2017, Allele-specific analysis of cell fusion-mediated pluripotent reprograming reveals distinct and predictive susceptibilities of human X-linked genes to reactivation, GENOME BIOLOGY, Vol: 18, ISSN: 1474-760X

JOURNAL ARTICLE

Cantone I, Fisher AG, 2017, Human X chromosome inactivation and reactivation: implications for cell reprogramming and disease, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 372, ISSN: 0962-8436

JOURNAL ARTICLE

Fisher AG, Stumpf MPH, Merkenschlager M, 2017, Reconciling Epigenetic Memory and Transcriptional Responsiveness, CELL SYSTEMS, Vol: 4, Pages: 373-374, ISSN: 2405-4712

JOURNAL ARTICLE

Fisher CL, Marks H, Cho LT-Y, Andrews R, Wormald S, Carroll T, Iyer V, Tate P, Rosen B, Stunnenberg HG, Fisher AG, Skarnes WCet al., 2017, An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers, NUCLEIC ACIDS RESEARCH, Vol: 45, ISSN: 0305-1048

JOURNAL ARTICLE

Liang Z, Brown KE, Carroll T, Taylor B, Vidal IF, Hendrich B, Rueda D, Fisher AG, Merkenschlager Met al., 2017, A high-resolution map of transcriptional repression, ELIFE, Vol: 6, ISSN: 2050-084X

JOURNAL ARTICLE

Van de Pette M, Abbas A, Feytout A, McNamara G, Bruno L, To WK, Dimond A, Sardini A, Webster Z, McGinty J, Paul EJ, Ungless MA, French PMW, Withers DJ, Uren A, Ferguson-Smith AC, Merkenschlager M, John RM, Fisher AGet al., 2017, Visualizing Changes in Cdkn1c Expression Links Early-Life Adversity to Imprint Mis-regulation in Adults, CELL REPORTS, Vol: 18, Pages: 1090-1099, ISSN: 2211-1247

JOURNAL ARTICLE

Cantone I, Bagci H, Dormann D, Dharmalingam G, Nesterova T, Brockdorff N, Rougeulle C, Vallot C, Heard E, Chaligne R, Merkenschlager M, Fisher AGet al., 2016, Ordered chromatin changes and human X chromosome reactivation by cell fusion-mediated pluripotent reprogramming, NATURE COMMUNICATIONS, Vol: 7, ISSN: 2041-1723

JOURNAL ARTICLE

Graham B, Marcais A, Dharmalingam G, Carroll T, Kanellopoulou C, Graumann J, Nesterova TB, Bermange A, Brazauskas P, Xella B, Kriaucionis S, Higgs DR, Brockdorff N, Mann M, Fisher AG, Merkenschlager Met al., 2016, MicroRNAs of the miR-290-295 Family Maintain Bivalency in Mouse Embryonic Stem Cells, STEM CELL REPORTS, Vol: 6, Pages: 635-642, ISSN: 2213-6711

JOURNAL ARTICLE

Gupta P, Lavagnolli T, Mira-Bontenbal H, Fisher AG, Merkenschlager Met al., 2016, Cohesin's role in pluripotency and reprogramming, CELL CYCLE, Vol: 15, Pages: 324-330, ISSN: 1538-4101

JOURNAL ARTICLE

Malinowski AR, Fisher AG, 2016, Reprogramming of Somatic Cells Towards Pluripotency by Cell Fusion., Pages: 289-299

Pluripotent reprogramming can be dominantly induced in a somatic nucleus upon fusion with a pluripotent cell such as embryonic stem (ES) cell. Cell fusion between ES cells and somatic cells results in the formation of heterokaryons, in which the somatic nuclei begin to acquire features of the pluripotent partner. The generation of interspecies heterokaryons between mouse ES- and human somatic cells allows an experimenter to distinguish the nuclear events occurring specifically within the reprogrammed nucleus. Therefore, cell fusion provides a simple and rapid approach to look at the early nuclear events underlying pluripotent reprogramming. Here, we describe a polyethylene glycol (PEG)-mediated cell fusion protocol to generate interspecies heterokaryons and intraspecies hybrids between ES cells and B lymphocytes or fibroblasts.

BOOK CHAPTER

Blevins R, Bruno L, Carroll T, Elliott J, Marcais A, Loh C, Hertweck A, Krek A, Rajewsky N, Chen C-Z, Fisher AG, Merkenschlager Met al., 2015, microRNAs Regulate Cell-to-Cell Variability of Endogenous Target Gene Expression in Developing Mouse Thymocytes, PLOS GENETICS, Vol: 11, ISSN: 1553-7390

JOURNAL ARTICLE

Chiu FWY, Bagci H, Fisher AG, deMello AJ, Elvira KSet al., 2015, A microfluidic toolbox for cell fusion, Journal of Chemical Technology and Biotechnology, Vol: 91, Pages: 16-24, ISSN: 1097-4660

Cellular fusion is a key process in many fields ranging from historical gene mapping studies and monoclonal antibody production, through to cell reprogramming. Traditional methodologies for cell fusion rely on the random pairing of different cell types and generally result in low and variable fusion efficiencies. These approaches become particularly limiting where substantial numbers of bespoke one-to-one fusions are required, for example, for in-depth studies of nuclear reprogramming mechanisms. In recent years, microfluidic technologies have proven valuable in creating platforms where the manipulation of single cells is highly efficient, rapid and controllable. These technologies also allow the integration of different experimental steps and characterisation processes into a single platform. Although the application of microfluidic methodologies to cell fusion studies is promising, current technologies that rely on static trapping are limited both in terms of the overall number of fused cells produced and their experimental accessibility. Here we review some of the most exciting breakthroughs in core microfluidic technologies that will allow the creation of integrated platforms for controlled cell fusion at high throughput. © 2015 Society of Chemical Industry.

JOURNAL ARTICLE

Ing-Simmons E, Seitan VC, Faure AJ, Flicek P, Carroll T, Dekker J, Fisher AG, Lenhard B, Merkenschlager Met al., 2015, Spatial enhancer clustering and regulation of enhancer-proximal genes by cohesin, GENOME RESEARCH, Vol: 25, Pages: 504-513, ISSN: 1088-9051

JOURNAL ARTICLE

Landeira D, Bagci H, Malinowski AR, Brown KE, Soza-Ried J, Feytout A, Webster Z, Ndjetehe E, Cantone I, Asenjo HG, Brockdorff N, Carroll T, Merkenschlager M, Fisher AGet al., 2015, Jarid2 Coordinates Nanog Expression and PCP/Wnt Signaling Required for Efficient ESC Differentiation and Early Embryo Development, CELL REPORTS, Vol: 12, Pages: 573-586, ISSN: 2211-1247

JOURNAL ARTICLE

Lavagnolli T, Gupta P, Hörmanseder E, Mira-Bontenbal H, Dharmalingam G, Carroll T, Gurdon JB, Fisher AG, Merkenschlager Met al., 2015, Initiation and maintenance of pluripotency gene expression in the absence of cohesin., Genes Dev, Vol: 29, Pages: 23-38

Cohesin is implicated in establishing and maintaining pluripotency. Whether this is because of essential cohesin functions in the cell cycle or in gene regulation is unknown. Here we tested cohesin's contribution to reprogramming in systems that reactivate the expression of pluripotency genes in the absence of proliferation (embryonic stem [ES] cell heterokaryons) or DNA replication (nuclear transfer). Contrary to expectations, cohesin depletion enhanced the ability of ES cells to initiate somatic cell reprogramming in heterokaryons. This was explained by increased c-Myc (Myc) expression in cohesin-depleted ES cells, which promoted DNA replication-dependent reprogramming of somatic fusion partners. In contrast, cohesin-depleted somatic cells were poorly reprogrammed in heterokaryons, due in part to defective DNA replication. Pluripotency gene induction was rescued by Myc, which restored DNA replication, and by nuclear transfer, where reprogramming does not require DNA replication. These results redefine cohesin's role in pluripotency and reveal a novel function for Myc in promoting the replication-dependent reprogramming of somatic nuclei.

JOURNAL ARTICLE

Marcais A, Blevins R, Graumann J, Feytout A, Dharmalingam G, Carroll T, Amado IF, Bruno L, Lee K, Walzer T, Mann M, Freitas AA, Boothby M, Fisher AG, Merkenschlager Met al., 2014, microRNA-mediated regulation of mTOR complex components facilitates discrimination between activation and anergy in CD4 T cells, JOURNAL OF EXPERIMENTAL MEDICINE, Vol: 211, Pages: 2281-2295, ISSN: 0022-1007

JOURNAL ARTICLE

Piccolo FM, Fisher AG, 2014, Getting rid of DNA methylation, TRENDS IN CELL BIOLOGY, Vol: 24, Pages: 136-143, ISSN: 0962-8924

JOURNAL ARTICLE

Bagci H, Fisher AG, 2013, DNA Demethylation in Pluripotency and Reprogramming: The Role of Tet Proteins and Cell Division, CELL STEM CELL, Vol: 13, Pages: 265-269, ISSN: 1934-5909

JOURNAL ARTICLE

Brown KE, Bagci H, Soza-Ried J, Fisher AGet al., 2013, Atypical heterochromatin organization and replication are rapidly acquired by somatic cells following fusion-mediated reprogramming by mouse ESCs, CELL CYCLE, Vol: 12, Pages: 3253-3261, ISSN: 1538-4101

JOURNAL ARTICLE

Cantone I, Fisher AG, 2013, Epigenetic programming and reprogramming during development, NATURE STRUCTURAL & MOLECULAR BIOLOGY, Vol: 20, Pages: 282-289, ISSN: 1545-9993

JOURNAL ARTICLE

Ferreiros-Vidal I, Carroll T, Taylor B, Terry A, Liang Z, Bruno L, Dharmalingam G, Khadayate S, Cobb BS, Smale ST, Spivakov M, Srivastava P, Petretto E, Fisher AG, Merkenschlager Met al., 2013, Genome-wide identification of Ikaros targets elucidates its contribution to mouse B-cell lineage specification and pre-B-cell differentiation, BLOOD, Vol: 121, Pages: 1769-1782, ISSN: 0006-4971

JOURNAL ARTICLE

Piccolo FM, Bagci H, Brown KE, Landeira D, Soza-Ried J, Feytout A, Mooijman D, Hajkova P, Leitch HG, Tada T, Kriaucionis S, Dawlaty MM, Jaenisch R, Merkenschlager M, Fisher AGet al., 2013, Different Roles for Tet1 and Tet2 Proteins in Reprogramming-Mediated Erasure of Imprints Induced by EGC Fusion, MOLECULAR CELL, Vol: 49, Pages: 1023-1033, ISSN: 1097-2765

JOURNAL ARTICLE

Piccolo FM, Bagci H, Brown KE, Landeira D, Soza-Ried J, Feytout A, Mooijman D, Hajkova P, Leitch HG, Tada T, Kriaucionis S, Dawlaty MM, Jaenisch R, Merkenschlager M, Fisher AGet al., 2013, Errata to Different roles for tet1 and tet2 proteins in reprogramming-mediated erasure of imprints induced by egc fusion [Molecular Cell, 49 (2013) 1023-1033], Molecular Cell, Vol: 49, ISSN: 1097-2765

JOURNAL ARTICLE

Seitan VC, Faure AJ, Zhan Y, McCord RP, Lajoie BR, Ing-Simmons E, Lenhard B, Giorgetti L, Heard E, Fisher AG, Flicek P, Dekker J, Merkenschlager Met al., 2013, Cohesin-based chromatin interactions enable regulated gene expression within preexisting architectural compartments, GENOME RESEARCH, Vol: 23, Pages: 2066-2077, ISSN: 1088-9051

JOURNAL ARTICLE

Tsubouchi T, Fisher AG, 2013, Reprogramming and the Pluripotent Stem Cell Cycle, Editors: Heard, Publisher: ELSEVIER ACADEMIC PRESS INC, Pages: 223-241, ISBN: 978-0-12-416027-9

BOOK CHAPTER

Tsubouchi T, Soza-Ried J, Brown K, Piccolo FM, Cantone I, Landeira D, Bagci H, Hochegger H, Merkenschlager M, Fisher AGet al., 2013, DNA Synthesis Is Required for Reprogramming Mediated by Stem Cell Fusion, CELL, Vol: 152, Pages: 873-883, ISSN: 0092-8674

JOURNAL ARTICLE

Fisher AG, Brockdorff N, 2012, Epigenetic memory and parliamentary privilege combine to evoke discussions on inheritance, DEVELOPMENT, Vol: 139, Pages: 3891-3896, ISSN: 0950-1991

JOURNAL ARTICLE

Merkenschlager M, Seitan V, Tachibana K, Hao B, Fisher AG, Nasmyth K, Krangel M, Merkenschlager Met al., 2012, Cohesin regulates T cell receptor rearrangement and thymocyte differentiation, 1st Biennial Symposium on Regulatory T Cells and T Helper Cells, Publisher: WILEY-BLACKWELL, Pages: 3-3, ISSN: 0019-2805

CONFERENCE PAPER

Soza-Ried J, Fisher AG, 2012, Reprogramming somatic cells towards pluripotency by cellular fusion, CURRENT OPINION IN GENETICS & DEVELOPMENT, Vol: 22, Pages: 459-465, ISSN: 0959-437X

JOURNAL ARTICLE

Cantone I, Fisher AG, 2011, Unraveling Epigenetic Landscapes: The Enigma of Enhancers, CELL STEM CELL, Vol: 8, Pages: 128-129, ISSN: 1934-5909

JOURNAL ARTICLE

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