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@article{Singh:2012:10.1002/0471140864.ps2903s67,
author = {Singh, S and Zhang, M and Bertheleme, N and Kara, E and Strange, PG and Byrne, B},
doi = {10.1002/0471140864.ps2903s67},
journal = {Curr Protoc Protein Sci},
pages = {29.3.1--29.3.22},
title = {Radioligand binding analysis as a tool for quality control of GPCR production for structural characterization: adenosine A(2a)R as a template for study.},
url = {http://dx.doi.org/10.1002/0471140864.ps2903s67},
volume = {Chapter 29},
year = {2012}
}

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TY  - JOUR
AB  - Functional characterization of G protein-coupled receptors is essential to ascertain the suitability of a protein target for downstream studies and to help develop optimal expression and isolation procedures. Radioligand binding analysis is a well-established technique, which allows direct measurement of the amount of functional receptor in a sample. It can be readily applied to both membrane-bound and soluble receptor samples and is an ideal method for monitoring the amount of functional protein at each stage in the expression and isolation process. This unit presents protocols for the radioligand binding analysis of the human adenosine A(2a) receptor and provides examples of how these assays can be used at several stages to help optimize expression, solubilization, and isolation procedures.
AU  - Singh,S
AU  - Zhang,M
AU  - Bertheleme,N
AU  - Kara,E
AU  - Strange,PG
AU  - Byrne,B
DO  - 10.1002/0471140864.ps2903s67
EP  - 3
PY  - 2012///
SP  - 29
TI  - Radioligand binding analysis as a tool for quality control of GPCR production for structural characterization: adenosine A(2a)R as a template for study.
T2  - Curr Protoc Protein Sci
UR  - http://dx.doi.org/10.1002/0471140864.ps2903s67
UR  - https://www.ncbi.nlm.nih.gov/pubmed/22294328
VL  - Chapter 29
ER  -

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