Imperial College London
Portrait Picture

DrClaraLopes Novo

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Fellow
 
 
 
//

Contact

 

c.lopes-novo

 
 
//

Location

 

Commonwealth BuildingHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Novo:2022:10.1038/s41467-022-31198-3,
author = {Novo, CL and Wong, E and Hockings, C and Poudel, C and Sheekey, E and Wiese, M and Okkenhaug, H and Boulton, SJ and Basu, S and Walker, S and Schierle, GSK and Narlikar, GJ and Rugg-Gunn, PJ},
doi = {10.1038/s41467-022-31198-3},
journal = {Nature Communications},
pages = {1--16},
title = {Satellite repeat transcripts modulate heterochromatin condensates and safeguard chromosome stability in mouse embryonic stem cells},
url = {http://dx.doi.org/10.1038/s41467-022-31198-3},
volume = {13},
year = {2022}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Heterochromatin maintains genome integrity and function, and is organised into distinct nuclear domains. Some of these domains are proposed to form by phase separation through the accumulation of HP1. Mouse heterochromatin contains noncoding major satellite repeats (MSR), which are highly transcribed in mouse embryonic stem cells (ESCs). Here, we report that MSR transcripts can drive the formation of HP1 droplets in vitro, and modulate heterochromatin into dynamic condensates in ESCs, contributing to the formation of large nuclear domains that are characteristic of pluripotent cells. Depleting MSR transcripts causes heterochromatin to transition into a more compact and static state. Unexpectedly, changing heterochromatin’s biophysical properties has severe consequences for ESCs, including chromosome instability and mitotic defects. These findings uncover an essential role for MSR transcripts in modulating the organisation and properties of heterochromatin to preserve genome stability. They also provide insights into the processes that could regulate phase separation and the functional consequences of disrupting the properties of heterochromatin condensates.
AU  - Novo,CL
AU  - Wong,E
AU  - Hockings,C
AU  - Poudel,C
AU  - Sheekey,E
AU  - Wiese,M
AU  - Okkenhaug,H
AU  - Boulton,SJ
AU  - Basu,S
AU  - Walker,S
AU  - Schierle,GSK
AU  - Narlikar,GJ
AU  - Rugg-Gunn,PJ
DO  - 10.1038/s41467-022-31198-3
EP  - 16
PY  - 2022///
SN  - 2041-1723
SP  - 1
TI  - Satellite repeat transcripts modulate heterochromatin condensates and safeguard chromosome stability in mouse embryonic stem cells
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-022-31198-3
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000813768100004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://www.nature.com/articles/s41467-022-31198-3
UR  - http://hdl.handle.net/10044/1/101827
VL  - 13
ER  -
Download