Imperial College London
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Professor David MacIntyre

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor in Reproduction Systems Medicine
 
 
 
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Contact

 

+44 (0)20 7594 2195d.macintyre Website

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mitra:2015:10.1038/srep16865,
author = {Mitra, A and MacIntyre, D and lee, YS and Smith, A and Marchesi, J and Lehne, B and Bhatia, R and lyons, D and Paraskevaidis, E and Li, J and holmes, E and nicholson, JK and bennett, PR and kyrgiou, M},
doi = {10.1038/srep16865},
journal = {Scientific Reports},
title = {Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity},
url = {http://dx.doi.org/10.1038/srep16865},
volume = {5},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Persistent infection with oncogenic Human Papillomavirus (HPV) is necessary for cervical carcinogenesis. Although evidence suggests that the vaginal microbiome plays a functional role in the persistence or regression of HPV infections, this has yet to be described in women with cervical intra-epithelial neoplasia (CIN). We hypothesised that increasing microbiome diversity is associated with increasing CIN severity. llumina MiSeq sequencing of 16S rRNA gene amplicons was used to characterise the vaginal microbiota of women with low-grade squamous intra-epithelial lesions (LSIL; n = 52), high-grade (HSIL; n = 92), invasive cervical cancer (ICC; n = 5) and healthy controls (n = 20). Hierarchical clustering analysis revealed an increased prevalence of microbiomes characterised by high-diversity and low levels of Lactobacillus spp. (community state type-CST IV) with increasing disease severity, irrespective of HPV status (Normal = 2/20,10%; LSIL = 11/52,21%; HSIL = 25/92,27%; ICC = 2/5,40%). Increasing disease severity was associated with decreasing relative abundance of Lactobacillus spp. The vaginal microbiome in HSIL was characterised by higher levels of Sneathia sanguinegens (P < 0.01), Anaerococcus tetradius (P < 0.05) and Peptostreptococcus anaerobius (P < 0.05) and lower levels of Lactobacillus jensenii (P < 0.01) compared to LSIL. Our results suggest advancing CIN disease severity is associated with increasing vaginal microbiota diversity and may be involved in regulating viral persistence and disease progression.
AU  - Mitra,A
AU  - MacIntyre,D
AU  - lee,YS
AU  - Smith,A
AU  - Marchesi,J
AU  - Lehne,B
AU  - Bhatia,R
AU  - lyons,D
AU  - Paraskevaidis,E
AU  - Li,J
AU  - holmes,E
AU  - nicholson,JK
AU  - bennett,PR
AU  - kyrgiou,M
DO  - 10.1038/srep16865
PY  - 2015///
SN  - 2045-2322
TI  - Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep16865
UR  - http://hdl.handle.net/10044/1/27743
VL  - 5
ER  -
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