Imperial College London
Portrait Picture

Professor David S. Rueda

Faculty of Medicine, Department of Infectious Disease

Chair in Molecular and Cellular Biophysics
 
 
 
//

Contact

 

david.rueda Website

 
 
//

Location

 

6.12DLMS BuildingHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Han:2023:10.1016/j.molcel.2023.08.007,
author = {Han, Z and Moore, GA and Mitter, R and Lopez, Martinez D and Wan, L and Dirac, Svejstrup AB and Rueda, DS and Svejstrup, JQ},
doi = {10.1016/j.molcel.2023.08.007},
journal = {Mol Cell},
pages = {3253--3267.e7},
title = {DNA-directed termination of RNA polymerase II transcription.},
url = {http://dx.doi.org/10.1016/j.molcel.2023.08.007},
volume = {83},
year = {2023}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - RNA polymerase II (RNAPII) transcription involves initiation from a promoter, transcriptional elongation through the gene, and termination in the terminator region. In bacteria, terminators often contain specific DNA elements provoking polymerase dissociation, but RNAPII transcription termination is thought to be driven entirely by protein co-factors. We used biochemical reconstitution, single-molecule studies, and genome-wide analysis in yeast to study RNAPII termination. Transcription into natural terminators by pure RNAPII results in spontaneous termination at specific sequences containing T-tracts. Single-molecule analysis indicates that termination involves pausing without backtracking. The "torpedo" Rat1-Rai1 exonuclease (XRN2 in humans) greatly stimulates spontaneous termination but is ineffectual on other paused RNAPIIs. By contrast, elongation factor Spt4-Spt5 (DSIF) suppresses termination. Genome-wide analysis further indicates that termination occurs by transcript cleavage at the poly(A) site exposing a new 5' RNA-end that allows Rat1-Rai1 loading, which then catches up with destabilized RNAPII at specific termination sites to end transcription.
AU  - Han,Z
AU  - Moore,GA
AU  - Mitter,R
AU  - Lopez,Martinez D
AU  - Wan,L
AU  - Dirac,Svejstrup AB
AU  - Rueda,DS
AU  - Svejstrup,JQ
DO  - 10.1016/j.molcel.2023.08.007
EP  - 3267
PY  - 2023///
SP  - 3253
TI  - DNA-directed termination of RNA polymerase II transcription.
T2  - Mol Cell
UR  - http://dx.doi.org/10.1016/j.molcel.2023.08.007
UR  - https://www.ncbi.nlm.nih.gov/pubmed/37683646
VL  - 83
ER  -
Download