Imperial College London
Alternate

ProfessorElaineHolmes

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Chemical Biology
 
 
 
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Contact

 

+44 (0)20 7594 3220elaine.holmes

 
 
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Location

 

661Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{James:2019:10.1038/s41598-019-51901-7,
author = {James, K and Bottacini, F and Contreras, JIS and Vigoureux, M and Egan, M and Motherway, MO and Holmes, E and van, Sinderen D},
doi = {10.1038/s41598-019-51901-7},
journal = {Scientific Reports},
pages = {1--20},
title = {Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal},
url = {http://dx.doi.org/10.1038/s41598-019-51901-7},
volume = {9},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A number of bifidobacterial species are found at a particularly high prevalence and abundance in faecal samples of healthy breastfed infants, a phenomenon that is believed to be, at least partially, due to the ability of bifidobacteria to metabolize Human Milk Oligosaccharides (HMOs). In the current study, we isolated a novel strain of Bifidobacterium kashiwanohense, named APCKJ1, from the faeces of a four-week old breastfed infant, based on the ability of the strain to utilise the HMO component fucosyllactose. We then determined the full genome sequence of this strain, and employed the generated data to analyze fucosyllactose metabolism in B. kashiwanohense APCKJ1. Transcriptomic and growth analyses, combined with metabolite analysis, in vitro hydrolysis assays and heterologous expression, allowed us to elucidate the pathway for fucosyllactose metabolism in B. kashiwanohense APCKJ1. Homologs of the key genes for this metabolic pathway were identified in particular in infant-derived members of the Bifdobacterium genus, revealing the apparent niche-specific nature of this pathway, and allowing a broad perspective on bifidobacterial fucosyllactose and L-fucose metabolism.
AU  - James,K
AU  - Bottacini,F
AU  - Contreras,JIS
AU  - Vigoureux,M
AU  - Egan,M
AU  - Motherway,MO
AU  - Holmes,E
AU  - van,Sinderen D
DO  - 10.1038/s41598-019-51901-7
EP  - 20
PY  - 2019///
SN  - 2045-2322
SP  - 1
TI  - Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-019-51901-7
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000492832300012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/s41598-019-51901-7
UR  - http://hdl.handle.net/10044/1/86610
VL  - 9
ER  -
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