Imperial College London
Alternate

ProfessorElaineHolmes

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Chemical Biology
 
 
 
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Contact

 

+44 (0)20 7594 3220elaine.holmes

 
 
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Location

 

661Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Claus:2011:10.1128/mBio.00271-10,
author = {Claus, SP and Ellero, SL and Berger, B and Krause, L and Bruttin, A and Molina, J and Paris, A and Want, EJ and de, Waziers I and Cloarec, O and Richards, SE and Wang, Y and Dumas, M-E and Ross, A and Rezzi, S and Kochhar, S and Van, Bladeren P and Lindon, JC and Holmes, E and Nicholson, JK},
doi = {10.1128/mBio.00271-10},
journal = {mBio},
pages = {e00271--e00210},
title = {Colonization-induced host-gut microbial metabolic interaction.},
url = {http://dx.doi.org/10.1128/mBio.00271-10},
volume = {2},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - UNLABELLED: The gut microbiota enhances the host's metabolic capacity for processing nutrients and drugs and modulate the activities of multiple pathways in a variety of organ systems. We have probed the systemic metabolic adaptation to gut colonization for 20 days following exposure of axenic mice (n = 35) to a typical environmental microbial background using high-resolution (1)H nuclear magnetic resonance (NMR) spectroscopy to analyze urine, plasma, liver, kidney, and colon (5 time points) metabolic profiles. Acquisition of the gut microbiota was associated with rapid increase in body weight (4%) over the first 5 days of colonization with parallel changes in multiple pathways in all compartments analyzed. The colonization process stimulated glycogenesis in the liver prior to triggering increases in hepatic triglyceride synthesis. These changes were associated with modifications of hepatic Cyp8b1 expression and the subsequent alteration of bile acid metabolites, including taurocholate and tauromuricholate, which are essential regulators of lipid absorption. Expression and activity of major drug-metabolizing enzymes (Cyp3a11 and Cyp2c29) were also significantly stimulated. Remarkably, statistical modeling of the interactions between hepatic metabolic profiles and microbial composition analyzed by 16S rRNA gene pyrosequencing revealed strong associations of the Coriobacteriaceae family with both the hepatic triglyceride, glucose, and glycogen levels and the metabolism of xenobiotics. These data demonstrate the importance of microbial activity in metabolic phenotype development, indicating that microbiota manipulation is a useful tool for beneficially modulating xenobiotic metabolism and pharmacokinetics in personalized health care. IMPORTANCE: Gut bacteria have been associated with various essential biological functions in humans such as energy harvest and regulation of blood pressure. Furthermore, gut microbial colonization occurs after birth in parallel with other
AU  - Claus,SP
AU  - Ellero,SL
AU  - Berger,B
AU  - Krause,L
AU  - Bruttin,A
AU  - Molina,J
AU  - Paris,A
AU  - Want,EJ
AU  - de,Waziers I
AU  - Cloarec,O
AU  - Richards,SE
AU  - Wang,Y
AU  - Dumas,M-E
AU  - Ross,A
AU  - Rezzi,S
AU  - Kochhar,S
AU  - Van,Bladeren P
AU  - Lindon,JC
AU  - Holmes,E
AU  - Nicholson,JK
DO  - 10.1128/mBio.00271-10
EP  - 00210
PY  - 2011///
SP  - 00271
TI  - Colonization-induced host-gut microbial metabolic interaction.
T2  - mBio
UR  - http://dx.doi.org/10.1128/mBio.00271-10
UR  - https://www.ncbi.nlm.nih.gov/pubmed/21363910
UR  - http://hdl.handle.net/10044/1/14523
VL  - 2
ER  -
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