Imperial College London
Alternate

ProfessorElaineHolmes

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Chemical Biology
 
 
 
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Contact

 

+44 (0)20 7594 3220elaine.holmes

 
 
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Location

 

661Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Anwar:2017:10.1038/s41598-017-02529-y,
author = {Anwar, MA and Adesina-Georgiadis, KN and Spagou, K and Vorkas, PA and Li, JV and Shalhoub, J and Holmes, E and Davies, AH},
doi = {10.1038/s41598-017-02529-y},
journal = {Scientific Reports},
title = {A comprehensive characterisation of the metabolic profile of varicose veins; implications in elaborating plausible cellular pathways for disease pathogenesis},
url = {http://dx.doi.org/10.1038/s41598-017-02529-y},
volume = {7},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Metabolic phenotypes reflect both the genetic and environmental factors which contribute to the development of varicose veins (VV). This study utilises analytical techniques to provide a comprehensive metabolic picture of VV disease, with the aim of identifying putative cellular pathways of disease pathogenesis. VV (n = 80) and non-VV (n = 35) aqueous and lipid metabolite extracts were analysed using 600 MHz 1H Nuclear Magnetic Resonance spectroscopy and Ultra-Performance Liquid Chromatography Mass Spectrometry. A subset of tissue samples (8 subjects and 8 controls) were analysed for microRNA expression and the data analysed with mirBase (www.mirbase.org). Using Multivariate statistical analysis, Ingenuity pathway analysis software, DIANALAB database and published literature, the association of significant metabolites with relevant cellular pathways were understood. Higher concentrations of glutamate, taurine, myo-inositol, creatine and inosine were present in aqueous extracts and phosphatidylcholine, phosphatidylethanolamine and sphingomyelin in lipid extracts in the VV group compared with non-VV group. Out of 7 differentially expressed miRNAs, spearman correlation testing highlighted correlation of hsa-miR-642a-3p, hsa-miR-4459 and hsa-miR-135a-3p expression with inosine in the vein tissue, while miR-216a-5p, conversely, was correlated with phosphatidylcholine and phosphatidylethanolamine. Pathway analysis revealed an association of phosphatidylcholine and sphingomyelin with inflammation and myo-inositol with cellular proliferation.
AU  - Anwar,MA
AU  - Adesina-Georgiadis,KN
AU  - Spagou,K
AU  - Vorkas,PA
AU  - Li,JV
AU  - Shalhoub,J
AU  - Holmes,E
AU  - Davies,AH
DO  - 10.1038/s41598-017-02529-y
PY  - 2017///
SN  - 2045-2322
TI  - A comprehensive characterisation of the metabolic profile of varicose veins; implications in elaborating plausible cellular pathways for disease pathogenesis
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-017-02529-y
UR  - http://hdl.handle.net/10044/1/48106
VL  - 7
ER  -
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