Imperial College London
Portrait Picture

ProfessorIolyKotta-Loizou

Faculty of Natural Sciences, Department of Life Sciences

Honorary Lecturer
 
 
 
//

Contact

 

i.kotta-loizou13

 
 
//

Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Hao:2022:10.1002/advs.202103669,
author = {Hao, M and Ye, F and Jovanovic, M and Kotta-Loizou, I and Xu, Q and Qin, X and Buck, M and Zhang, X and Wang, M},
doi = {10.1002/advs.202103669},
journal = {Advanced Science},
pages = {1--10},
title = {Structures of Class I and Class II transcription complexes reveal the molecular basis of RamA-dependent transcription activation},
url = {http://dx.doi.org/10.1002/advs.202103669},
volume = {9},
year = {2022}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Transcription activator RamA is linked to multidrug resistance of Klebsiella pneumoniae through controlling genes that encode efflux pumps (acrA) and porin-regulating antisense RNA (micF). In bacteria, σ70, together with activators, controls the majority of genes by recruiting RNA polymerase (RNAP) to the promoter regions. RNAP and σ70 form a holoenzyme that recognizes -35 and -10 promoter DNA consensus sites. Many activators bind upstream from the holoenzyme and can be broadly divided into two classes. RamA acts as a class I activator on acrA and class II activator on micF, respectively. The authors present biochemical and structural data on RamA in complex with RNAP-σ70 at the two promoters and the data reveal the molecular basis for how RamA assembles and interacts with core RNAP and activates transcription that contributes to antibiotic resistance. Further, comparing with CAP/TAP complexes reveals common and activator-specific features in activator binding and uncovers distinct roles of the two C-terminal domains of RNAP α subunit.
AU  - Hao,M
AU  - Ye,F
AU  - Jovanovic,M
AU  - Kotta-Loizou,I
AU  - Xu,Q
AU  - Qin,X
AU  - Buck,M
AU  - Zhang,X
AU  - Wang,M
DO  - 10.1002/advs.202103669
EP  - 10
PY  - 2022///
SN  - 2198-3844
SP  - 1
TI  - Structures of Class I and Class II transcription complexes reveal the molecular basis of RamA-dependent transcription activation
T2  - Advanced Science
UR  - http://dx.doi.org/10.1002/advs.202103669
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000716835900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://onlinelibrary.wiley.com/doi/10.1002/advs.202103669
UR  - http://hdl.handle.net/10044/1/92981
VL  - 9
ER  -
Download