Imperial College London
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DrJavierAlegre Abarrategui

Faculty of Medicine, Department of Brain Sciences

Clinical Senior Lecturer in Neuropathology
 
 
 
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Contact

 

+44 (0)20 7594 6683j.alegre

 
 
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Assistant

 

Mrs Hadeel Abdeen +44 (0)20 7594 7014

 
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Location

 

E416Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Phadwal:2012:10.4161/auto.18935,
author = {Phadwal, K and Alegre-Abarrategui, J and Watson, AS and Pike, L and Anbalagan, S and Hammond, EM and Wade-Martins, R and McMichael, A and Klenerman, P and Simon, AK},
doi = {10.4161/auto.18935},
journal = {Autophagy},
pages = {677--689},
title = {A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells},
url = {http://dx.doi.org/10.4161/auto.18935},
volume = {8},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Autophagy is a conserved constitutive cellular process, responsible for the degradation of dysfunctional proteins and organelles. Autophagy plays a role in many diseases such as neurodegeneration and cancer; however, to date, conventional autophagy detection techniques are not suitable for clinical samples. We have developed a high throughput, statistically robust technique that quantitates autophagy in primary human leukocytes using the Image stream, an imaging flow cytometer. We validate this method on cell lines and primary cells knocked down for essential autophagy genes. Also, using this method we show that T cells have higher autophagic activity than B cells. Furthermore our results indicate that healthy primary senescent CD8+ T cells have decreased autophagic levels correlating with increased DNA damage, which may explain features of the senescent immune system and its declining function with age. This technique will allow us, for the first time, to measure autophagy levels in diseases with a known link to autophagy, while also determining the contribution of autophagy to the efficacy of drugs.
AU  - Phadwal,K
AU  - Alegre-Abarrategui,J
AU  - Watson,AS
AU  - Pike,L
AU  - Anbalagan,S
AU  - Hammond,EM
AU  - Wade-Martins,R
AU  - McMichael,A
AU  - Klenerman,P
AU  - Simon,AK
DO  - 10.4161/auto.18935
EP  - 689
PY  - 2012///
SN  - 1554-8627
SP  - 677
TI  - A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells
T2  - Autophagy
UR  - http://dx.doi.org/10.4161/auto.18935
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000305403400012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/61094
VL  - 8
ER  -
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