Imperial College London
Alternate

ProfessorJuliaGorelik

Faculty of Medicine, National Heart & Lung Institute

Professor of Cellular Biophysics
 
 
 
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Contact

 

+44 (0)20 7594 2736j.gorelik Website

 
 
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Assistant

 

Miss Cheryl Costello +44 (0)20 7594 3001

 
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Location

 

429ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ruenraroengsak:2011:10.3109/17435390.2011.558643,
author = {Ruenraroengsak, P and Novak, P and Berhanu, D and Thorley, AJ and Valsami-Jones, E and Gorelik, J and Korchev, YE and Tetley, TD},
doi = {10.3109/17435390.2011.558643},
journal = {Nanotoxicology},
title = {Respiratory epithelial cytotoxicity and membrane damage (holes) caused by amine-modified nanoparticles.},
url = {http://dx.doi.org/10.3109/17435390.2011.558643},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Abstract The respiratory epithelium is a significant target of inhaled, nano-sized particles, the biological reactivity of which will depend on its physicochemical properties. Surface-modified, 50 and 100 nm, polystyrene latex nanoparticles (NPs) were used as model particles to examine the effect of particle size and surface chemistry on transformed human alveolar epithelial type 1-like cells (TT1). Live images of TT1 exposed to amine-modified NPs taken by hopping probe ion conductance microscopy revealed severe damage and holes on cell membranes that were not observed with other types of NPs. This paralleled induction of cell detachment, cytotoxicity and apoptotic (caspase-3/7 and caspase-9) cell death, and increased release of CXCL8 (IL-8). In contrast, unmodified, carboxyl-modified 50 nm NPs and the 100 nm NPs did not cause membrane damage, and were less reactive. Thus, the susceptibility and membrane damage to respiratory epithelium following inhalation of NPs will depend on both surface chemistry (e.g., cationic) and nano-size.
AU  - Ruenraroengsak,P
AU  - Novak,P
AU  - Berhanu,D
AU  - Thorley,AJ
AU  - Valsami-Jones,E
AU  - Gorelik,J
AU  - Korchev,YE
AU  - Tetley,TD
DO  - 10.3109/17435390.2011.558643
PY  - 2011///
SN  - 1743-5404
TI  - Respiratory epithelial cytotoxicity and membrane damage (holes) caused by amine-modified nanoparticles.
T2  - Nanotoxicology
UR  - http://dx.doi.org/10.3109/17435390.2011.558643
UR  - http://www.ncbi.nlm.nih.gov/pubmed/21352086
ER  -
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