Imperial College London
Alternate

Emeritus ProfessorJeremyNicholson

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Emeritus Professor of Biological Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 3195j.nicholson Website

 
 
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Assistant

 

Ms Wendy Torto +44 (0)20 7594 3225

 
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Location

 

Office no. 665Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wist:2023:clinchem/hvad097.194,
author = {Wist, J and Nitschke, P and Masuda, R and Lodge, S and Schaefer, H and Cannet, C and Holmes, E and Nicholson, JK},
doi = {clinchem/hvad097.194},
journal = {Clinical Chemistry},
title = {A-216 Measuring Inflammation and Cardiovascular Markers at Benchtop NMR using Diffusion and Relaxation Edited Experiments},
url = {http://dx.doi.org/10.1093/clinchem/hvad097.194},
volume = {69},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title>               <jats:sec>                  <jats:title>Background</jats:title>                  <jats:p>Metabolic phenotyping is an established tool in systems medicine that captures a profile of one’s individual health status and reflects the interaction between genes and external stressors. It uses analytical platforms such as NMR or MS to acquire molecular profiles, and modelling to extract actionable knowledge. Applying these techniques to a cohort of Australians infected by SARS-CoV-2 revealed a strong alteration in regions of 1D NMR spectra associated with lipoproteins and glycoproteins, and referred as Supramolecular Phospholipid Composite SPC (δ = 3.2 ppm) and Glyc (δ = 2.07 ppm). The latter is an established marker of inflammation. These results were later confirmed using larger cohorts from Spain (n = 525) and the UK (n = 1022). The urgent need for very rapid testing, at the early stage of the pandemic, prompted the development of bespoke NMR experiments able to measure this lipoproteins/glycoproteins signature without requiring complex modelling.</jats:p>               </jats:sec>               <jats:sec>                  <jats:title>Methods</jats:title>                  <jats:p>Physico-chemical properties of lipoprotein particles, such as diffusion, transverse and longitudinal relaxation rates, differ from low molecular weight metabolites. Therefore, an edited experiment JEDI (PGPE) was designed that combines diffusion, relaxation and scalar coupling editing blocks to produce a lipoprotein profile devoid of chemical noise (overlapping peaks) and where peaks give quantitative results.</jats:p>               </jats:sec>               <jats:sec>                  <jats:title>Results</jats:title>                  <jats:p>The SPC peak was further broken down into 3 sub-regions that are related to the mai
AU  - Wist,J
AU  - Nitschke,P
AU  - Masuda,R
AU  - Lodge,S
AU  - Schaefer,H
AU  - Cannet,C
AU  - Holmes,E
AU  - Nicholson,JK
DO  - clinchem/hvad097.194
PY  - 2023///
SN  - 0009-9147
TI  - A-216 Measuring Inflammation and Cardiovascular Markers at Benchtop NMR using Diffusion and Relaxation Edited Experiments
T2  - Clinical Chemistry
UR  - http://dx.doi.org/10.1093/clinchem/hvad097.194
VL  - 69
ER  -
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