Imperial College London
Alternate

ProfessorJesusGil

Faculty of Medicine, Institute of Clinical Sciences

Professor of Cell Proliferation
 
 
 
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Contact

 

+44 (0)20 3313 8263jesus.gil

 
 
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Location

 

ICTEM room 230ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gil:2005:10.1158/0008-5472.CAN-03-4030,
author = {Gil, J and Kerai, P and Lleonart, M and Bernard, D and Cigudosa, JC and Peters, G and Carnero, A and Beach, DH},
doi = {10.1158/0008-5472.CAN-03-4030},
journal = {Cancer Res},
pages = {2179--85},
title = {Immortalization of primary human prostate epithelial cells by c-Myc},
url = {http://dx.doi.org/10.1158/0008-5472.CAN-03-4030},
volume = {65},
year = {2005}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A significant percentage of prostate tumors have amplifications of the c-Myc gene, but the precise role of c-Myc in prostate cancer is not fully understood. Immortalization of human epithelial cells involves both inactivation of the Rb/p16INK4a pathway and telomere maintenance, and it has been recapitulated in culture by expression of the catalytic subunit of telomerase, hTERT, in combination with viral oncoproteins. Here, we show the immortalization of human prostate epithelial cells (HPrEC) by a single genetic event, the expression of the c-Myc oncogene. Myc stabilizes telomere length in HPrEC through up-regulation of hTERT expression and overrides the accumulation of cell cycle inhibitory proteins, such as p16INK4a. Overall, HPrECs expressing c-Myc retain many characteristics of normal cells, such as the induction of a senescence-like growth arrest in response to oncogenic Ras, an intact p53 response, and an absence of gross karyotypic abnormalities. However, HPrECs expressing c-Myc lack a Rb/p16INK4a checkpoint and can be transformed without the need for additional genetic lesions in that pathway. These results give a partial explanation for the physiologic role of c-Myc overexpression in prostate cancer.
AU  - Gil,J
AU  - Kerai,P
AU  - Lleonart,M
AU  - Bernard,D
AU  - Cigudosa,JC
AU  - Peters,G
AU  - Carnero,A
AU  - Beach,DH
DO  - 10.1158/0008-5472.CAN-03-4030
EP  - 85
PY  - 2005///
SP  - 2179
TI  - Immortalization of primary human prostate epithelial cells by c-Myc
T2  - Cancer Res
UR  - http://dx.doi.org/10.1158/0008-5472.CAN-03-4030
VL  - 65
ER  -
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