Imperial College London
Portrait Picture

Dr Joseph van Batenburg-Sherwood

Faculty of Engineering, Department of Bioengineering

Lecturer
 
 
 
//

Contact

 

+44 (0)20 7594 7244jvbsherwood Website

 
 
//

Location

 

Uren 416ASir Michael Uren HubWhite City Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Kaliviotis:2016:10.3233/CH-151980,
author = {Kaliviotis, E and Dusting, J and Sherwood, JM and Balabani, S},
doi = {10.3233/CH-151980},
journal = {Clinical Hemorheology and Microcirculation},
pages = {123--148},
title = {Quantifying local characteristics of velocity, aggregation and hematocrit of human erythrocytes in a microchannel flow},
url = {http://dx.doi.org/10.3233/CH-151980},
volume = {63},
year = {2016}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The effect of erythrocyte aggregation on blood viscosity and microcirculatory flow is a poorly understood area of haemodynamics, especially with relevance to serious pathological conditions. Advances in microfluidics have made it possible to study the details of blood flow in the microscale, however, important issues such as the relationship between the local microstructure and local flow characteristics have not been investigated extensively. In the present study an experimental system involving simple brightfield microscopy has been successfully developed for simultaneous, time-resolved quantification of velocity fields and local aggregation of human red blood cells (RBC) in microchannels. RBCs were suspended in Dextran and phosphate buffer saline solutions for the control of aggregation. Local aggregation characteristics were investigated at bulk and local levels using statistical and edge-detection image processing techniques. A special case of aggregating flow in a microchannel, in which hematocrit gradients were present, was studied as a function of flowrate and time. The level of aggregation was found to strongly correlate with local variations in velocity in both the bulk flow and wall regions. The edge detection based analysis showed that near the side wall large aggregates are associated with regions corresponding to high local velocities and low local shear. On the contrary, in the bulk flow region large aggregates occurred in regions of low velocity and high erythrocyte concentration suggesting a combined effect of haematocrit and velocity distributions on local aggregation characteristics. The results of this study showed that using multiple methods for aggregation quantification, albeit empirical, could help towards a robust characterisation of the structural properties of the fluid.
AU  - Kaliviotis,E
AU  - Dusting,J
AU  - Sherwood,JM
AU  - Balabani,S
DO  - 10.3233/CH-151980
EP  - 148
PY  - 2016///
SN  - 1875-8622
SP  - 123
TI  - Quantifying local characteristics of velocity, aggregation and hematocrit of human erythrocytes in a microchannel flow
T2  - Clinical Hemorheology and Microcirculation
UR  - http://dx.doi.org/10.3233/CH-151980
UR  - http://hdl.handle.net/10044/1/34128
VL  - 63
ER  -
Download