Summary
Summary
My PhD and postdoctoral work have focused on the study of barrier immunity, with a particular emphasis on the signals regulating the local establishment of protective T cell responses, in both infectious and inflammatory settings.
As a PhD student in the laboratories of Dr. Shabaana Khader and Dr. Jay Kolls at the University of Pittsburgh, my studies focused on the factors required for the induction of local protective immune responses against Mycobacterium tuberculosis (Mtb) and other pulmonary mycobacterial pathogens. I subsequently joined Dr. Sarah Gaffen’s lab at the University of Pittsburgh for a postdoc. There, I continued my interest in barrier immunity, focusing now on skin inflammation. I found that the endoribonuclease MCPIP1 is a regulator of IL-17A and IL-17F signalling in the skin.
In 2016, I moved to London, where I joined Professor Adrian Hayday’s lab for a postdoc. Specialising now on gd T cells, I described a population of IL-17-producing uterine gd T cells which play a non-redundant role in the protection of the female reproductive tract against fungal infection. More recently, I began to query whether there are general rules governing how gd T cells are targeted to specific tissues. By optimising gene transfer methods for the study of the T cell receptor, I demonstrated that conserved mechanisms underlie the interaction between gd T cells and the epithelium across different organs (manuscript under preparation).
Research interests
Our research focuses on gd T cells, which are disproportionately enriched at body surfaces. gd T cells emerge from the thymus precommitted to an effector fate and are independent from MHC for their activation. Taken together, these features make them attractive for cancer immunotherapy and indeed, several gd T cell-based products have entered clinical trials. We study how specific gd T cell populations are established in tissues, including the gut and the female reproductive tract, and the impact of the bidirectional convesations that they engage in with the epithelium. Understanding the mechanisms underlying the developmental associations of gd T cells with tissues could offer basic biological insights into tissue immunosurveillance and improved therapeutic approaches applicable to many anatomical sites.
Publications
Trypanosoma cruzi-Derived Molecules Induce Anti-Tumour Protection by Favouring Both Innate and Adaptive Immune Responses.
Freire T, Landeira M, Giacomini C, Festari MF, Pittini Á, Cardozo V, Brosque A, Monin L, da Costa V, Faral-Tello P, Robello C, Osinaga E. Int J Mol Sci. 2022 Nov 30;23(23):15032. doi: 10.3390/ijms232315032. PMID: 36499361
Joseph M, Wu Y, Dannebaum R, Rubelt F, Zlatareva I, Lorenc A, Du ZG, Davies D, Kyle-Cezar F, Das A, Gee S, Seow J, Graham C, Telman D, Bermejo C, Lin H, Asgharian H, Laing AG, Del Molino Del Barrio I, Monin L, Muñoz-Ruiz M, McKenzie DR, Hayday TS, Francos-Quijorna I, Kamdar S, Davis R, Sofra V, Cano F, Theodoridis E, Martinez L, Merrick B, Bisnauthsing K, Brooks K, Edgeworth J, Cason J, Mant C, Doores KJ, Vantourout P, Luong K, Berka J, Hayday AC. Proc Natl Acad Sci U S A. 2022 Aug 23;119(34):e2201541119. doi: 10.1073/pnas.2201541119. Epub 2022 Aug 9. PMID: 35943978
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).
Cossarizza A, Chang HD, Radbruch A, Abrignani S, Addo R, Akdis M, Andrä I, Andreata F, Annunziato F, Arranz E, Bacher P, Bari S, Barnaba V, Barros-Martins J, Baumjohann D, Beccaria CG, Bernardo D, Boardman DA, Borger J, Böttcher C, Brockmann L, Burns M, Busch DH, Cameron G, Cammarata I, Cassotta A, Chang Y, Chirdo FG, Christakou E, Čičin-Šain L, Cook L, Corbett AJ, Cornelis R, Cosmi L, Davey MS, De Biasi S, De Simone G, Del Zotto G, Delacher M, Di Rosa F, Di Santo J, Diefenbach A, Dong J, Dörner T, Dress RJ, Dutertre CA, Eckle SBG, Eede P, Evrard M, Falk CS, Feuerer M, Fillatreau S, Fiz-Lopez A, Follo M, Foulds GA, Fröbel J, Gagliani N, Galletti G, Gangaev A, Garbi N, Garrote JA, Geginat J, Gherardin NA, Gibellini L, Ginhoux F, Godfrey DI, Gruarin P, Haftmann C, Hansmann L, Harpur CM, Hayday AC, Heine G, Hernández DC, Herrmann M, Hoelsken O, Huang Q, Huber S, Huber JE, Huehn J, Hundemer M, Hwang WYK, Iannacone M, Ivison SM, Jäck HM, Jani PK, Keller B, Kessler N, Ketelaars S, Knop L, Knopf J, Koay HF, Kobow K, Kriegsmann K, Kristyanto H, Krueger A, Kuehne JF, Kunze-Schumacher H, Kvistborg P, Kwok I, Latorre D, Lenz D, Levings MK, Lino AC, Liotta F, Long HM, Lugli E, MacDonald KN, M… Eur J Immunol. 2021 Dec;51(12):2708-3145. doi: 10.1002/eji.202170126. Epub 2021 Dec 7. PMID: 34910301
McKenzie DR, Muñoz-Ruiz M, Monin L, Alaguthurai T, Lechmere T, Abdul-Jawad S, Graham C, Pollock E, Graham R, Sychowska K, Seow J, Tremain C, Gousis C, Domingo-Vila C, Cooper J, Vidler J, Owczarczyk K, Swampillai A, Kristeleit H, Malim MH, Fields P, Patten PEM, Papa S, North BV, Tree T, Doores KJ, Hayday AC, Irshad S. Cancer Cell. 2021 Nov 8;39(11):1445-1447. doi: 10.1016/j.ccell.2021.10.003. Epub 2021 Oct 12. PMID: 34678151
Monin L, Laing AG, Muñoz-Ruiz M, McKenzie DR, Del Molino Del Barrio I, Alaguthurai T, Domingo-Vila C, Hayday TS, Graham C, Seow J, Abdul-Jawad S, Kamdar S, Harvey-Jones E, Graham R, Cooper J, Khan M, Vidler J, Kakkassery H, Sinha S, Davis R, Dupont L, Francos Quijorna I, O'Brien-Gore C, Lee PL, Eum J, Conde Poole M, Joseph M, Davies D, Wu Y, Swampillai A, North BV, Montes A, Harries M, Rigg A, Spicer J, Malim MH, Fields P, Patten P, Di Rosa F, Papa S, Tree T, Doores KJ, Hayday AC, Irshad S. Lancet Oncol. 2021 Jun;22(6):765-778. doi: 10.1016/S1470-2045(21)00213-8. Epub 2021 Apr 27. PMID: 33930323
Abdul-Jawad S, Baù L, Alaguthurai T, Del Molino Del Barrio I, Laing AG, Hayday TS, Monin L, Muñoz-Ruiz M, McDonald L, Francos Quijorna I, McKenzie D, Davis R, Lorenc A, Chan JNE, Ryan S, Bugallo-Blanco E, Yorke R, Kamdar S, Fish M, Zlatareva I, Vantourout P, Jennings A, Gee S, Doores K, Bailey K, Hazell S, De Naurois J, Moss C, Russell B, Khan AA, Rowley M, Benjamin R, Enting D, Alrifai D, Wu Y, Zhou Y, Barber P, Ng T, Spicer J, Van Hemelrijck M, Kumar M, Vidler J, Lwin Y, Fields P, Karagiannis SN, Coolen ACC, Rigg A, Papa S, Hayday AC, Patten PEM, Irshad S. Cancer Cell. 2021 Feb 8;39(2):257-275.e6. doi: 10.1016/j.ccell.2021.01.001. Epub 2021 Jan 5. PMID: 33476581
β2 Integrins differentially regulate γδ T cell subset thymic development and peripheral maintenance.
McIntyre CL, Monin L, Rop JC, Otto TD, Goodyear CS, Hayday AC, Morrison VL. Proc Natl Acad Sci U S A. 2020 Sep 8;117(36):22367-22377. doi: 10.1073/pnas.1921930117. Epub 2020 Aug 26. PMID: 32848068
A dynamic COVID-19 immune signature includes associations with poor prognosis.
Laing AG, Lorenc A, Del Molino Del Barrio I, Das A, Fish M, Monin L, Muñoz-Ruiz M, McKenzie DR, Hayday TS, Francos-Quijorna I, Kamdar S, Joseph M, Davies D, Davis R, Jennings A, Zlatareva I, Vantourout P, Wu Y, Sofra V, Cano F, Greco M, Theodoridis E, Freedman JD, Gee S, Chan JNE, Ryan S, Bugallo-Blanco E, Peterson P, Kisand K, Haljasmägi L, Chadli L, Moingeon P, Martinez L, Merrick B, Bisnauthsing K, Brooks K, Ibrahim MAA, Mason J, Lopez Gomez F, Babalola K, Abdul-Jawad S, Cason J, Mant C, Seow J, Graham C, Doores KJ, Di Rosa F, Edgeworth J, Shankar-Hari M, Hayday AC. Nat Med. 2020 Oct;26(10):1623-1635. doi: 10.1038/s41591-020-1038-6. Epub 2020 Aug 17. PMID: 32807934
Zhou C, Monin L, Gordon R, Aggor FEY, Bechara R, Edwards TN, Kaplan DH, Gingras S, Gaffen SL. J Immunol. 2020 Aug 1;205(3):720-730. doi: 10.4049/jimmunol.2000394. Epub 2020 Jun 29. PMID: 32601099
Jandke A, Melandri D, Monin L, Ushakov DS, Laing AG, Vantourout P, East P, Nitta T, Narita T, Takayanagi H, Feederle R, Hayday A. Nat Commun. 2020 Jul 28;11(1):3769. doi: 10.1038/s41467-020-17557-y. PMID: 32724083
Monin L, Ushakov DS, Arnesen H, Bah N, Jandke A, Muñoz-Ruiz M, Carvalho J, Joseph S, Almeida BC, Green MJ, Nye E, Hatano S, Yoshikai Y, Curtis M, Carlsen H, Steinhoff U, Boysen P, Hayday A. Mucosal Immunol. 2020 Nov;13(6):969-981. doi: 10.1038/s41385-020-0305-7. Epub 2020 May 29. PMID: 32472066
Immune responses in the human female reproductive tract.
Monin L, Whettlock EM, Male V. Immunology. 2020 Jun;160(2):106-115. doi: 10.1111/imm.13136. Epub 2019 Nov 11. PMID: 31630394
Monin L, Mehta S, Elsegeiny W, Gopal R, McAleer JP, Oury TD, Kolls J, Khader SA. Infect Immun. 2018 Feb 20;86(3):e00859-17. doi: 10.1128/IAI.00859-17. Print 2018 Mar. PMID: 29263106
Monin L, Gaffen SL. Cold Spring Harb Perspect Biol. 2018 Apr 2;10(4):a028522. doi: 10.1101/cshperspect.a028522. PMID: 28620097
MCPIP1/Regnase-1 Restricts IL-17A- and IL-17C-Dependent Skin Inflammation.
Monin L, Gudjonsson JE, Childs EE, Amatya N, Xing X, Verma AH, Coleman BM, Garg AV, Killeen M, Mathers A, Ward NL, Gaffen SL. J Immunol. 2017 Jan 15;198(2):767-775. doi: 10.4049/jimmunol.1601551. Epub 2016 Dec 5. PMID: 27920272
Kumar P, Monin L, Castillo P, Elsegeiny W, Horne W, Eddens T, Vikram A, Good M, Schoenborn AA, Bibby K, Montelaro RC, Metzger DW, Gulati AS, Kolls JK. Immunity. 2016 Mar 15;44(3):659-671. doi: 10.1016/j.immuni.2016.02.007. PMID: 26982366
Helminth-induced arginase-1 exacerbates lung inflammation and disease severity in tuberculosis.
Monin L, Griffiths KL, Lam WY, Gopal R, Kang DD, Ahmed M, Rajamanickam A, Cruz-Lagunas A, Zúñiga J, Babu S, Kolls JK, Mitreva M, Rosa BA, Ramos-Payan R, Morrison TE, Murray PJ, Rangel-Moreno J, Pearce EJ, Khader SA. J Clin Invest. 2015 Dec;125(12):4699-713. doi: 10.1172/JCI77378. Epub 2015 Nov 16. PMID: 26571397
Immune requirements for protective Th17 recall responses to Mycobacterium tuberculosis challenge.
Monin L, Griffiths KL, Slight S, Lin Y, Rangel-Moreno J, Khader SA. Mucosal Immunol. 2015 Sep;8(5):1099-109. doi: 10.1038/mi.2014.136. Epub 2015 Jan 28. PMID: 25627812
Chemokines in tuberculosis: the good, the bad and the ugly.
Monin L, Khader SA. Semin Immunol. 2014 Dec;26(6):552-8. doi: 10.1016/j.smim.2014.09.004. Epub 2014 Oct 22. PMID: 25444549
Gopal R, Monin L, Slight S, Uche U, Blanchard E, Fallert Junecko BA, Ramos-Payan R, Stallings CL, Reinhart TA, Kolls JK, Kaushal D, Nagarajan U, Rangel-Moreno J, Khader SA. PLoS Pathog. 2014 May 15;10(5):e1004099. doi: 10.1371/journal.ppat.1004099. eCollection 2014 May. PMID: 24831696
Mycobacterium tuberculosis impairs dendritic cell functions through the serine hydrolase Hip1.
Madan-Lala R, Sia JK, King R, Adekambi T, Monin L, Khader SA, Pulendran B, Rengarajan J. J Immunol. 2014 May 1;192(9):4263-72. doi: 10.4049/jimmunol.1303185. Epub 2014 Mar 21. PMID: 24659689
S100A8/A9 proteins mediate neutrophilic inflammation and lung pathology during tuberculosis.
Gopal R, Monin L, Torres D, Slight S, Mehra S, McKenna KC, Fallert Junecko BA, Reinhart TA, Kolls J, Báez-Saldaña R, Cruz-Lagunas A, Rodríguez-Reyna TS, Kumar NP, Tessier P, Roth J, Selman M, Becerril-Villanueva E, Baquera-Heredia J, Cumming B, Kasprowicz VO, Steyn AJ, Babu S, Kaushal D, Zúñiga J, Vogl T, Rangel-Moreno J, Khader SA. Am J Respir Crit Care Med. 2013 Nov 1;188(9):1137-46. doi: 10.1164/rccm.201304-0803OC. PMID: 24047412
Antitumor activity of human hydatid cyst fluid in a murine model of colon cancer.
Berriel E, Russo S, Monin L, Festari MF, Berois N, Fernández G, Freire T, Osinaga E. ScientificWorldJournal. 2013 Aug 18;2013:230176. doi: 10.1155/2013/230176. eCollection 2013. PMID: 24023528
Slight SR, Monin L, Gopal R, Avery L, Davis M, Cleveland H, Oury TD, Rangel-Moreno J, Khader SA. Am J Pathol. 2013 Nov;183(5):1397-1404. doi: 10.1016/j.ajpath.2013.07.008. Epub 2013 Sep 3. PMID: 24007881
Ho AW, Garg AV, Monin L, Simpson-Abelson MR, Kinner L, Gaffen SL. PLoS One. 2013 Jul 29;8(7):e70168. doi: 10.1371/journal.pone.0070168. Print 2013. PMID: 23922952
Monin L, Khader SA. Am J Respir Crit Care Med. 2013 Jun 1;187(11):1162-4. doi: 10.1164/rccm.201303-0552ED. PMID: 23725611