Imperial College London
Alternate

DrLauraNolan

Faculty of Medicine, National Heart & Lung Institute

Honorary Research Fellow
 
 
 
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Contact

 

l.nolan

 
 
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Location

 

G45Emmanuel Kaye BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hynen:2021:10.1099/mic.0.000990,
author = {Hynen, AL and Lazenby, JJ and Savva, GM and McCaughey, LC and Turnbull, L and Nolan, LM and Whitchurch, CB},
doi = {10.1099/mic.0.000990},
journal = {Microbiology},
pages = {1--12},
title = {Multiple holins contribute to extracellular DNA release in Pseudomonas aeruginosa biofilms},
url = {http://dx.doi.org/10.1099/mic.0.000990},
volume = {167},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bacterial biofilms are composed of aggregates of cells encased within a matrix of extracellular polymeric substances (EPS). One key EPS component is extracellular DNA (eDNA), which acts as a ‘glue’, facilitating cell–cell and cell–substratum interactions. We have previously demonstrated that eDNA is produced in Pseudomonas aeruginosa biofilms via explosive cell lysis. This phenomenon involves a subset of the bacterial population explosively lysing, due to peptidoglycan degradation by the endolysin Lys. Here we demonstrate that in P. aeruginosa three holins, AlpB, CidA and Hol, are involved in Lys-mediated eDNA release within both submerged (hydrated) and interstitial (actively expanding) biofilms, albeit to different extents, depending upon the type of biofilm and the stage of biofilm development. We also demonstrate that eDNA release events determine the sites at which cells begin to cluster to initiate microcolony formation during the early stages of submerged biofilm development. Furthermore, our results show that sustained release of eDNA is required for cell cluster consolidation and subsequent microcolony development in submerged biofilms. Overall, this study adds to our understanding of how eDNA release is controlled temporally and spatially within P. aeruginosa biofilms.
AU  - Hynen,AL
AU  - Lazenby,JJ
AU  - Savva,GM
AU  - McCaughey,LC
AU  - Turnbull,L
AU  - Nolan,LM
AU  - Whitchurch,CB
DO  - 10.1099/mic.0.000990
EP  - 12
PY  - 2021///
SN  - 1350-0872
SP  - 1
TI  - Multiple holins contribute to extracellular DNA release in Pseudomonas aeruginosa biofilms
T2  - Microbiology
UR  - http://dx.doi.org/10.1099/mic.0.000990
UR  - https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000990
UR  - http://hdl.handle.net/10044/1/87104
VL  - 167
ER  -
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