Imperial College London
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DrMariaPapathanasiou

Faculty of Engineering, Department of Chemical Engineering

Senior Lecturer
 
 
 
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Contact

 

maria.papathanasiou11

 
 
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Location

 

RODH.501DRoderic Hill BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Velliou:2015:10.1007/s00449-015-1401-z,
author = {Velliou, EG and Brito, dos Santos SUSANA and Papathanasiou, MM and Fuentes-Gari, M and Misener, R and Panoskaltsis, N and Pistikopoulos, EN and Mantalaris, A},
doi = {10.1007/s00449-015-1401-z},
journal = {Bioprocess and Biosystems Engineering: bioreactors, upstream and downstream processes, measurement and control},
pages = {1589--1600},
title = {Towards unravelling the kinetics of an acute myeloid leukaemia model system under oxidative and starvation stress: a comparison between two- and three-dimensional cultures},
url = {http://dx.doi.org/10.1007/s00449-015-1401-z},
volume = {38},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A great challenge when conducting ex vivo studies of leukaemia is the construction of an appropriate experimental platform that would recapitulate the bone marrow (BM) environment. Such a 3D scaffold system has been previously developed in our group [1]. Additionally to the BM architectural characteristics, parameters such as oxygen and glucose concentration are crucial as their value could differ between patients as well as within the same patient at different stages of treatment, consequently affecting the resistance of leukaemia to chemotherapy. The effect of oxidative and glucose stress—at levels close to human physiologic ones—on the proliferation and metabolic evolution of an AML model system (K-562 cell line) in conventional 2D cultures as well as in 3D scaffolds were studied. We observed that the K-562 cell line can proliferate and remain alive for 2 weeks in medium with glucose close to physiological levels both in 20 and 5 % O2. We report interesting differences on the cellular response to the environmental, i.e., oxidative and/or nutritional stress stimuli in 2D and 3D. Higher adaptation to oxidative stress under non-starving conditions is observed in the 3D system. The glucose level in the medium has more impact on the cellular proliferation in the 3D compared to the 2D system. These differences can be of significant importance both when applying chemotherapy in vitro and also when constructing mathematical tools for optimisation of disease treatment.
AU  - Velliou,EG
AU  - Brito,dos Santos SUSANA
AU  - Papathanasiou,MM
AU  - Fuentes-Gari,M
AU  - Misener,R
AU  - Panoskaltsis,N
AU  - Pistikopoulos,EN
AU  - Mantalaris,A
DO  - 10.1007/s00449-015-1401-z
EP  - 1600
PY  - 2015///
SN  - 0178-515X
SP  - 1589
TI  - Towards unravelling the kinetics of an acute myeloid leukaemia model system under oxidative and starvation stress: a comparison between two- and three-dimensional cultures
T2  - Bioprocess and Biosystems Engineering: bioreactors, upstream and downstream processes, measurement and control
UR  - http://dx.doi.org/10.1007/s00449-015-1401-z
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000358934700017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/87435
VL  - 38
ER  -
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