Imperial College London
Alternate

DrNicholasKirkby

Faculty of Medicine, National Heart & Lung Institute

Senior Research Fellow
 
 
 
//

Contact

 

+44 (0)20 7594 3075n.kirkby

 
 
//

Location

 

364Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Lee:2018:10.1016/j.phymed.2017.12.003,
author = {Lee, S-Y and Chang, W-L and Li, Z-X and Kirkby, NS and Tsai, W-C and Huang, S-F and Ou, C-H and Chang, T-C},
doi = {10.1016/j.phymed.2017.12.003},
journal = {PHYTOMEDICINE},
pages = {183--191},
title = {Astragaloside VI and cycloastragenol-6-O-beta-D-glucoside promote wound healing in vitro and in vivo},
url = {http://dx.doi.org/10.1016/j.phymed.2017.12.003},
volume = {38},
year = {2018}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundAstragalus genus includes most of the common, historical herbal medicines that have various applications in Asian countries. However, clinical data and mechanistic insights into their actions are still lacking.PurposeIn this study, we aimed to examine the effects of astragalosides on wound healing in vitro and in vivo, as well as the underlying mechanisms of these actions.MethodsThe wound healing activity of astragalosides was investigated in human HaCaT keratinocytes, human dermal fibroblast (HDF) cells, and murine models of wound healing.ResultsAll eight astragalosides studied enhanced epidermal growth factor receptor (EGFR) activity in HaCaT cells. Among them, astragaloside VI (AS-VI) showed the strongest EGFR activation. Consistently, AS-VI and cycloastragenol-6-O-beta-D-glucoside (CMG), which is the major metabolite of astragalosides, enhanced extracellular signal-regulated kinase (ERK) activity in a concentration-dependent manner. In agreement, both compounds induced EGFR-dependent cell proliferation and migration in HaCaT and HDF cells. In addition, we showed that AS-VI and CMG accelerated the healing of both sterile and infected wounds in vivo. These effects were associated with increased angiogenesis in the scar tissue.ConclusionAS-VI and CMG increased the proliferation and migration of skin cells via activation of the EGFR/ERK signalling pathway, resulting in the improvement of wound healing in vitro and in vivo. These findings indicate the therapeutic potential of AS-VI and CMG to accelerate wound healing; additionally, they suggest the mechanistic basis of this activity.
AU  - Lee,S-Y
AU  - Chang,W-L
AU  - Li,Z-X
AU  - Kirkby,NS
AU  - Tsai,W-C
AU  - Huang,S-F
AU  - Ou,C-H
AU  - Chang,T-C
DO  - 10.1016/j.phymed.2017.12.003
EP  - 191
PY  - 2018///
SN  - 0944-7113
SP  - 183
TI  - Astragaloside VI and cycloastragenol-6-O-beta-D-glucoside promote wound healing in vitro and in vivo
T2  - PHYTOMEDICINE
UR  - http://dx.doi.org/10.1016/j.phymed.2017.12.003
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000425172900020&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/61918
VL  - 38
ER  -
Download