Imperial College London
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Dr Tolga Bozkurt

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Plant-Microbe
 
 
 
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Contact

 

+44 (0)20 7594 5381o.bozkurt

 
 
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Location

 

6167Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Adachi:2019:10.1101/693291,
author = {Adachi, H and Contreras, M and Harant, A and Wu, C-H and Derevnina, L and Sakai, T and Duggan, C and Moratto, E and Bozkurt, TO and Maqbool, A and Win, J and Kamoun, S},
doi = {10.1101/693291},
title = {An N-terminal motif in NLR immune receptors is functionally conserved across distantly related plant species},
url = {http://dx.doi.org/10.1101/693291},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:p>The molecular codes underpinning the functions of plant NLR immune receptors are poorly understood. We used <jats:italic>in vitro</jats:italic> Mu transposition to generate a random truncation library and identify the minimal functional region of NLRs. We applied this method to NRC4—a helper NLR that functions with multiple sensor NLRs within a Solanaceae receptor network. This revealed that the NRC4 N-terminal 29 amino acids are sufficient to induce hypersensitive cell death. This region is defined by the consensus MADAxVSFxVxKLxxLLxxEx (MADA motif) that is conserved at the N-termini of NRC family proteins and ~20% of coiled-coil (CC)-type plant NLRs. The MADA motif matches the N-terminal α1 helix of Arabidopsis NLR protein ZAR1, which undergoes a conformational switch during resistosome activation. Immunoassays revealed that the MADA motif is functionally conserved across NLRs from distantly related plant species. NRC-dependent sensor NLRs lack MADA sequences indicating that this motif has degenerated in sensor NLRs over evolutionary time.</jats:p>
AU  - Adachi,H
AU  - Contreras,M
AU  - Harant,A
AU  - Wu,C-H
AU  - Derevnina,L
AU  - Sakai,T
AU  - Duggan,C
AU  - Moratto,E
AU  - Bozkurt,TO
AU  - Maqbool,A
AU  - Win,J
AU  - Kamoun,S
DO  - 10.1101/693291
PY  - 2019///
TI  - An N-terminal motif in NLR immune receptors is functionally conserved across distantly related plant species
UR  - http://dx.doi.org/10.1101/693291
ER  -
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