Imperial College London
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ProfessorPaulFrench

Faculty of Natural Sciences, Department of Physics

Professor of Physics
 
 
 
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Contact

 

+44 (0)20 7594 7706paul.french Website

 
 
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Assistant

 

Ms Judith Baylis +44 (0)20 7594 7713

 
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Location

 

609Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@inbook{Dunsby:2014:10.1201/b17290,
author = {Dunsby, C and McGinty, J and French, P},
booktitle = {Biomedical Photonics Handbook, Second Edition: Fundamentals, Devices, and Techniques},
doi = {10.1201/b17290},
pages = {531--560},
title = {Multidimensional fluorescence imaging of biological tissue},
url = {http://dx.doi.org/10.1201/b17290},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - CHAP
AB  - This chapter aims to review multidimensional fluorescence imaging (MDFI) technology and its application to biological tissue, with a particular emphasis on fluorescence lifetime imaging (FLIM) of biological tissue with examples from our work at Imperial College London. Fluorescence imaging is flourishing tremendously, partly driven by advances in laser and detector technology, partly by advances in labeling technologies such as genetically expressed fluorescent proteins, and partly by advances in computational analysis techniques. Increasingly, fluorescence instrumentation is developed to provide more information than just the localization or distribution of specific fluorescent molecules. Often, fluorescence signals are analyzed to provide information on the local fluorophore environment or to contrast different fluorophores in complex mixtures-as often occur in biological tissue. This trend to higher-content fluorescence imaging increasingly exploits MDFI and measurement capabilities with instrumentation that resolves fluorescence lifetime together with other spectroscopic parameters such as excitation and emission wavelength and polarization, providing image information in two or three spatial dimensions as well as with respect to elapsed time (Figure 18.1). However, caution should be exercised when acquiring such MDFI since photobleaching or experimental considerations usually impose a limited photon budget and/or a maximum image acquisition time and also present significant challenges with respect to data analysis and data management. These considerations are particularly important for real-time clinical diagnostic applications, for higher-throughput assays, and for the investigation of dynamic biological systems (Figure 18.1).
AU  - Dunsby,C
AU  - McGinty,J
AU  - French,P
DO  - 10.1201/b17290
EP  - 560
PY  - 2014///
SN  - 9781420085129
SP  - 531
TI  - Multidimensional fluorescence imaging of biological tissue
T1  - Biomedical Photonics Handbook, Second Edition: Fundamentals, Devices, and Techniques
UR  - http://dx.doi.org/10.1201/b17290
ER  -
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