Imperial College London
Alternate

DrVassoTerzidou

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Clinical Reader in Obstetrics and Gynaecology
 
 
 
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Contact

 

+44 (0)20 7594 2164v.terzidou

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Khanjani:2010:10.1111/j.1582-4934.2010.01069.x,
author = {Khanjani, S and Kandola, MK and Lindstrom, TM and Sooranna, SR and Melchionda, M and Lee, YS and Terzidou, V and Johnson, MR and Bennett, PR},
doi = {10.1111/j.1582-4934.2010.01069.x},
journal = {Journal of Cellular and Molecular Medicine},
pages = {809--824},
title = {NF-kB regulates a cassette of immune/inflammatory genes in human pregnant myometrium at term},
url = {http://dx.doi.org/10.1111/j.1582-4934.2010.01069.x},
volume = {15},
year = {2010}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The onset of human labour resembles inflammation with increased synthesis of prostaglandins and cytokines. There is evidence from rodent models for an important role for nuclear factor-κB (NF-κB) activity in myometrium which both up-regulates contraction-associated proteins and antagonizes the relaxatory effects of progesterone. Here we show that in the human, although there are no differences in expression of NF-κB p65, or IκB-α between upper- or lower-segment myometrium or before or after labour, there is nuclear localization of serine-256-phospho-p65 and serine-536-phospho-p65 in both upper- and lower-segment myometrium both before and after the onset of labour at term. This shows that NF-κB is active in both upper and lower segment prior to the onset of labour at term. To identify the range of genes regulated by NF-κB we overexpressed p65 in myocytes in culture. This led to NF-κB activation identical to that seen following interleukin (IL)-1β stimulation, including phosphorylation and nuclear translocation of p65 and p50. cDNA microarray analysis showed that NF-κB increased expression of 38 genes principally related to immunity and inflammation. IL-1β stimulation also resulted in an increase in the expression of the same genes. Transfection with siRNA against p65 abolished the response to IL-1β proving a central role for NF-κB. We conclude that NF-κB is active in myocytes in both the upper and lower segment of the uterus prior to the onset of labour at term and principally regulates a group of immune/inflammation associated genes, demonstrating that myocytes can act as immune as well as contractile cells.
AU  - Khanjani,S
AU  - Kandola,MK
AU  - Lindstrom,TM
AU  - Sooranna,SR
AU  - Melchionda,M
AU  - Lee,YS
AU  - Terzidou,V
AU  - Johnson,MR
AU  - Bennett,PR
DO  - 10.1111/j.1582-4934.2010.01069.x
EP  - 824
PY  - 2010///
SN  - 1582-4934
SP  - 809
TI  - NF-kB regulates a cassette of immune/inflammatory genes in human pregnant myometrium at term
T2  - Journal of Cellular and Molecular Medicine
UR  - http://dx.doi.org/10.1111/j.1582-4934.2010.01069.x
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000290312700010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/43508
VL  - 15
ER  -
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