Imperial College London
Portrait Picture

DrYanLiu

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer in Glycosciences
 
 
 
//

Contact

 

yan.liu2 Website

 
 
//

Location

 

E518Burlington DanesHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Shahin:2023:10.1073/pnas.2216237120,
author = {Shahin, M and Sheppard, D and Raynaud, C and Berry, J and Gurung, I and Silva, L and Feizi, T and Liu, Y and Pelicic, V},
doi = {10.1073/pnas.2216237120},
journal = {Proceedings of the National Academy of Sciences of USA},
title = {Characterization of a glycan-binding complex of minor pilins completes theanalysis of Streptococcus sanguinis type 4 pili subunits},
url = {http://dx.doi.org/10.1073/pnas.2216237120},
volume = {120},
year = {2023}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Type 4 filaments (T4F)—of which type 4 pili (T4P) are the archetype—are a superfamily of nanomachines nearly ubiquitous in prokaryotes. T4F are polymers of one major pilin, which also contain minor pilins whose roles are often poorly understood. Here, we complete the structure/function analysis of the full set of T4P pilins in the opportunistic bacterial pathogen Streptococcus sanguinis. We determined the structure of the minor pilin PilA, which is unexpectedly similar to one of the subunits of a tip-located complex of four minor pilins, widely conserved in T4F. We found that PilA interacts and dramatically stabilizes the minor pilin PilC. We determined the structure of PilC, showing that it is a modular pilin with a lectin module binding a subset of glycans prevalent in the human glycome, the host of S. sanguinis. Altogether, our findings support a model whereby the minor pilins in S. sanguinis T4P form a tip-located complex promoting adhesion to various host receptors. This has general implications for T4F.
AU  - Shahin,M
AU  - Sheppard,D
AU  - Raynaud,C
AU  - Berry,J
AU  - Gurung,I
AU  - Silva,L
AU  - Feizi,T
AU  - Liu,Y
AU  - Pelicic,V
DO  - 10.1073/pnas.2216237120
PY  - 2023///
SN  - 0027-8424
TI  - Characterization of a glycan-binding complex of minor pilins completes theanalysis of Streptococcus sanguinis type 4 pili subunits
T2  - Proceedings of the National Academy of Sciences of USA
UR  - http://dx.doi.org/10.1073/pnas.2216237120
UR  - http://hdl.handle.net/10044/1/102185
VL  - 120
ER  -
Download