Imperial College London
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DrYanLiu

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer in Glycosciences
 
 
 
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Contact

 

yan.liu2 Website

 
 
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Location

 

E518Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Watson:2011,
author = {Watson, AA and Lebedev, AA and Hall, BA and Fenton-May, AE and Vagin, AA and Dejnirattisai, W and Felce, J and Mongkolsapaya, J and Palma, AS and Liu, Y and Feizi, T and Screaton, GR and Murshudov, GN and O'Callaghan, CA},
journal = {J.Biol.Chem.},
pages = {24208--24218},
title = {Structural flexibility of the macrophage dengue virus receptor CLEC5A: implications for ligand binding and signaling},
url = {pm:21566123},
volume = {286},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The human C-type lectin-like molecule CLEC5A is a critical macrophage receptor for dengue virus. The binding of dengue virus to CLEC5A triggers signaling through the associated adapter molecule DAP12, stimulating proinflammatory cytokine release. We have crystallized an informative ensemble of CLEC5A structural conformers at 1.9-A resolution and demonstrate how an on-off extension to a beta-sheet acts as a binary switch regulating the flexibility of the molecule. This structural information together with molecular dynamics simulations suggests a mechanism whereby extracellular events may be transmitted through the membrane and influence DAP12 signaling. We demonstrate that CLEC5A is homodimeric at the cell surface and binds to dengue virus serotypes 1-4. We used blotting experiments, surface analyses, glycan microarray, and docking studies to investigate the ligand binding potential of CLEC5A with particular respect to dengue virus. This study provides a rational foundation for understanding the dengue virus-macrophage interaction and the role of CLEC5A in dengue virus-induced lethal disease
AU  - Watson,AA
AU  - Lebedev,AA
AU  - Hall,BA
AU  - Fenton-May,AE
AU  - Vagin,AA
AU  - Dejnirattisai,W
AU  - Felce,J
AU  - Mongkolsapaya,J
AU  - Palma,AS
AU  - Liu,Y
AU  - Feizi,T
AU  - Screaton,GR
AU  - Murshudov,GN
AU  - O'Callaghan,CA
EP  - 24218
PY  - 2011///
SP  - 24208
TI  - Structural flexibility of the macrophage dengue virus receptor CLEC5A: implications for ligand binding and signaling
T2  - J.Biol.Chem.
UR  - pm:21566123
VL  - 286
ER  -
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