BibTex format
@article{Winterhalter:2023:nar/gkad277,
author = {Winterhalter, C and Pelliciari, S and Stevens, D and Fenyk, S and Marchand, E and Cronin, NB and Soultanas, P and Costa, TRD and Ilangovan, A and Murray, H},
doi = {nar/gkad277},
journal = {Nucleic Acids Research},
pages = {4322--4340},
title = {The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin},
url = {http://dx.doi.org/10.1093/nar/gkad277},
volume = {51},
year = {2023}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Genome replication is a fundamental biological activity shared by all organisms. Chromosomal replication proceeds bidirectionally from origins, requiring the loading of two helicases, one for each replisome. However, the molecular mechanisms underpinning helicase loading at bacterial chromosome origins (oriC) are unclear. Here we investigated the essential DNA replication initiation protein DnaD in the model organism Bacillus subtilis. A set of DnaD residues required for ssDNA binding was identified, and photo-crosslinking revealed that this ssDNA binding region interacts preferentially with one strand of oriC. Biochemical and genetic data support the model that DnaD recognizes a new single-stranded DNA (ssDNA) motif located in oriC, the DnaD Recognition Element (DRE). Considered with single particle cryo-electron microscopy (cryo-EM) imaging of DnaD, we propose that the location of the DRE within oriC orchestrates strand-specific recruitment of helicase during DNA replication initiation. These findings significantly advance our mechanistic understanding of bidirectional replication from a bacterial chromosome origin.
AU - Winterhalter,C
AU - Pelliciari,S
AU - Stevens,D
AU - Fenyk,S
AU - Marchand,E
AU - Cronin,NB
AU - Soultanas,P
AU - Costa,TRD
AU - Ilangovan,A
AU - Murray,H
DO - nar/gkad277
EP - 4340
PY - 2023///
SN - 0305-1048
SP - 4322
TI - The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin
T2 - Nucleic Acids Research
UR - http://dx.doi.org/10.1093/nar/gkad277
UR - https://academic.oup.com/nar/article/51/9/4322/7137456
UR - http://hdl.handle.net/10044/1/104240
VL - 51
ER -