(Picture courtesy - Kyasha Sri Ranjan: Example of widefield deconvolution)

Applications

• Deconvolution of widefield, confocal and multiphoton data
• Object analysis
• Colocalization analysis
• Charged Software

Access

From March 15^th, access the FILM Huygens software package will be different. Huygens will be run on a dedicated Windows server, which is more powerful than the current system. This should increase the speed for deconvolution by a factor of up to three.

 Please contact film-service if you require access or training of the Huygens software

Quick Start Guide - Huygens (PDF)

Saving as Tiff from Huygens (PDF)

Useful Youtube Videos

• Huygens Microscopic Parameter Wizard
• Huygens Batch Processor
• Huygens PSF Distiller
• Huygens Colocalization Analyzer
• Huygens Interactive Object Analyzer

• Huygens Deconvolution Express

Useful Webinars

• True Deconvolution Webinar
• Huygens Object Analyzer Webinar

Help, training and online information

• Free Huygens suite software viewer download (via Scientifice Volume Imaging website)
• Scientific Volume Imagine WIKI - very good source of information about formation of microscopy images and deconvolution (via Scientific Volume Imaging website)
• Nyquist calculator, to calculate the ideal settings for X, Y and Z-step size, magnification and zoom. Check before acquiring the data