Imperial College London
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DrMorganBeeby

Faculty of Natural Sciences, Department of Life Sciences

Reader in Structural Biology
 
 
 
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m.beeby Website

 
 
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Location

 

502Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Asmar:2017:10.1371/journal.pbio.2004303,
author = {Asmar, AT and Ferreira, JL and Cohen, EJ and Cho, S-H and Beeby, M and Hughes, KT and Collet, J-F},
doi = {10.1371/journal.pbio.2004303},
journal = {PLOS BIOLOGY},
title = {Communication across the bacterial cell envelope depends on the size of the periplasm},
url = {http://dx.doi.org/10.1371/journal.pbio.2004303},
volume = {15},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Thecellenvelopeof gram-negativebacteria,a structurecomprisinganouter(OM)andaninner(IM)membrane,is essentialforlife.TheOMandtheIMareseparatedbytheperi-plasm,a compartmentthatcontainsthepeptidoglycan. TheOMis tetheredto thepeptido-glycanviathelipoprotein,Lpp.However,theimportanceof theenvelope’smultilayeredarchitectureremainsunknown.Here,whenweremovedphysicalcouplingbetweentheOMandthepeptidoglycan,cellslosttheabilityto sensedefectsin envelopeintegrity.Furtherexperimentsrevealedthatthecriticalparameterforthetransmissionof stresssignalsfromtheenvelopeto thecytoplasm, wherecellularbehaviouris controlled,is theIM-to-OMdis-tance.Augmentingthisdistancebyincreasingthelengthof thelipoproteinLppdestroyedsignalling,whereassimultaneouslyincreasingthelengthof thestress-sensinglipoproteinRcsFrestoredsignalling.Ourresultsdemonstratethephysiological importanceof thesizeof theperiplasm.TheyalsorevealthatstrictcontrolovertheIM-to-OMdistanceis requiredforeffectiveenvelopesurveillanceandprotection,suggestingthatcellulararchitectureandthestructureof transenvelopeproteincomplexeshavebeenevolutionarilyco-optimisedforcorrectfunction.Similarstrategiesarelikelyat playin cellularcompartmentssurroundedby2 concentricmembranes,suchaschloroplastsandmitochondria.
AU  - Asmar,AT
AU  - Ferreira,JL
AU  - Cohen,EJ
AU  - Cho,S-H
AU  - Beeby,M
AU  - Hughes,KT
AU  - Collet,J-F
DO  - 10.1371/journal.pbio.2004303
PY  - 2017///
SN  - 1545-7885
TI  - Communication across the bacterial cell envelope depends on the size of the periplasm
T2  - PLOS BIOLOGY
UR  - http://dx.doi.org/10.1371/journal.pbio.2004303
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000418943900022&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/57385
VL  - 15
ER  -
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