Imperial College London
Alternate

DrArkhatAbzhanov

Faculty of Natural Sciences, Department of Life Sciences (Silwood Park)

Reader in Evolution and Developmental Genetics
 
 
 
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Contact

 

a.abzhanov

 
 
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Location

 

Munro 2.15MunroSilwood Park

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Summary

 

Publications

Citation

BibTex format

@article{Namkoong:2015:10.1016/j.actbio.2015.12.004,
author = {Namkoong, B and Guven, S and Ramesan, S and Liaudanskaya, V and Abzhanov, A and Demirci, U},
doi = {10.1016/j.actbio.2015.12.004},
journal = {Acta Biomaterialia},
pages = {301--311},
title = {Recapitulating cranial osteogenesis with neural crest cells in 3-D microenvironments},
url = {http://dx.doi.org/10.1016/j.actbio.2015.12.004},
volume = {31},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The experimental systems that recapitulate the complexity of native tissues and enable precise control over the microenvironment are becoming essential for the pre-clinical tests of therapeutics and tissue engineering. Here, we described a strategy to develop an in vitro platform to study the developmental biology of craniofacial osteogenesis. In this study, we directly osteo-differentiated cranial neural crest cells (CNCCs) in a 3-D in vitro bioengineered microenvironment. Cells were encapsulated in the gelatin-based photo-crosslinkable hydrogel and cultured up to three weeks. We demonstrated that this platform allows efficient differentiation of p75 positive CNCCs to cells expressing osteogenic markers corresponding to the sequential developmental phases of intramembranous ossification. During the course of culture, we observed a decrease in the expression of early osteogenic marker Runx2, while the other mature osteoblast and osteocyte markers such as Osterix, Osteocalcin, Osteopontin and Bone sialoprotein increased. We analyzed the ossification of the secreted matrix with alkaline phosphatase and quantified the newly secreted hydroxyapatite. The Field Emission Scanning Electron Microscope (FESEM) images of the bioengineered hydrogel constructs revealed the native-like osteocytes, mature osteoblasts, and cranial bone tissue morphologies with canaliculus-like intercellular connections. This platform provides a broadly applicable model system to potentially study diseases involving primarily embryonic craniofacial bone disorders, where direct diagnosis and adequate animal disease models are limited.
AU  - Namkoong,B
AU  - Guven,S
AU  - Ramesan,S
AU  - Liaudanskaya,V
AU  - Abzhanov,A
AU  - Demirci,U
DO  - 10.1016/j.actbio.2015.12.004
EP  - 311
PY  - 2015///
SN  - 1878-7568
SP  - 301
TI  - Recapitulating cranial osteogenesis with neural crest cells in 3-D microenvironments
T2  - Acta Biomaterialia
UR  - http://dx.doi.org/10.1016/j.actbio.2015.12.004
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000370086100027&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/42644
VL  - 31
ER  -
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