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@inproceedings{McKay:2010:10.1002/hep.23994,
author = {McKay, S and Unger, K and Sriraksa, R and Zeller, C and Pericleous, S and Limpaiboon, T and Hutchins, R and Spalding, D and Brown, B and Thomas, G},
doi = {10.1002/hep.23994},
title = {Differing copy number alteration profiles identified by array CGH in Thai IntrahepaticCholangiocarcinoma related to prognosis},
url = {http://dx.doi.org/10.1002/hep.23994},
year = {2010}
}

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TY  - CPAPER
AB  - Purpose: The worldwide incidence of intrahepatic cholangiocarcinomahas vastly increased over the past three decades,and now represents the second most common primary liver cancer.Despite this increase in incidence mortality figures forcholangiocarcinoma remain dismal. We employed array comparativegenomic hybridization to identify copy number alterationsin cholangiocarcinoma with prognostic significance.Methods: DNA was extracted from 24 Thai cases of intrahepaticcholangiocarcinoma (ICC), and labelled using randomprimer labelling (Enzo Life Sciences CGH Labeling Kit). TumourDNA was labelled with Cy3 and sex-mismatched referenceDNA (pooled normal DNA, Promega) with Cy5 fluorescencedye and hybridised to 180K Agilent Oligonucleotide arrayCGH slides for 24 hours. Microarray slides were scanned at 3micron (Agilent), fluorescence intensities extracted using theFeature Extraction Software (Agilent) and subsequentlyanalysed within the statistical environment R using the CBSalgorithm for segmenation of profiles and the CGHcall packagefor calling of copy number gains and losses. Results: Analysisof the data revealed three distinct groups of copy number (CN)alterations related to overall survival. Groups were separatedinto poor (>4 weeks and <13 weeks, n=9), moderate (>14weeks and <57 weeks, n=7) and good (>58 weeks, n=8) survival.The good survival group demonstrated very few CN alterations.Gains at 1q and 11q, and losses at 1p and 2q werespecific to poor survival. Gains at 1p, 7p and 13p/q, andlosses at 11p, 12q and 15p were specific for moderate survival.Several CN alterations were demonstrated in the poorand moderate group, and not found in the good group, includinggain at 2q, and losses at 3p, 4p/q, 5q, 6q, 8p and18p/q. The two most charateristic alterations were gain at1q21.1-24.2 in poor suvivors (p value < 0.04 FDR < 0.11) andgain at 8q24.3 in good survivors (p value < 0.001 and FDR=0.024). Conclusion: Intrahepatic cholangiocarcinoma remainsa clin
AU  - McKay,S
AU  - Unger,K
AU  - Sriraksa,R
AU  - Zeller,C
AU  - Pericleous,S
AU  - Limpaiboon,T
AU  - Hutchins,R
AU  - Spalding,D
AU  - Brown,B
AU  - Thomas,G
DO  - 10.1002/hep.23994
PY  - 2010///
TI  - Differing copy number alteration profiles identified by array CGH in Thai IntrahepaticCholangiocarcinoma related to prognosis
UR  - http://dx.doi.org/10.1002/hep.23994
UR  - http://onlinelibrary.wiley.com/doi/10.1002/hep.23994/abstract
ER  -

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