Imperial College London
Alternate

Professor George K. Christophides

Faculty of Natural Sciences, Department of Life Sciences

Professor of Infectious Diseases & Immunity
 
 
 
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Contact

 

+44 (0)20 7594 5342g.christophides

 
 
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Location

 

6165Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Dembo:2015:10.1186/s13071-015-0700-1,
author = {Dembo, EG and Abay, SM and Dahiya, N and Ogboi, JS and Christophides, GK and Lupidi, G and Chianese, G and Lucantoni, L and Habluetzel, A},
doi = {10.1186/s13071-015-0700-1},
journal = {Parasit Vectors},
title = {Impact of repeated NeemAzal-treated blood meals on the fitness of Anopheles stephensi mosquitoes.},
url = {http://dx.doi.org/10.1186/s13071-015-0700-1},
volume = {8},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Herbal remedies are widely used in many malaria endemic countries to treat patients, in particular in the absence of anti-malarial drugs and in some settings to prevent the disease. Herbal medicines may be specifically designed for prophylaxis and/or for blocking malaria transmission to benefit both, the individual consumer and the community at large. Neem represents a good candidate for this purpose due to its inhibitory effects on the parasite stages that cause the clinical manifestations of malaria and on those responsible for infection in the vector. Furthermore, neem secondary metabolites have been shown to interfere with various physiological processes in insect vectors. This study was undertaken to assess the impact of the standardised neem extract NeemAzal on the fitness of the malaria vector Anopheles stephensi following repeated exposure to the product through consecutive blood meals on treated mice. METHODS: Batches of An. stephensi mosquitoes were offered 5 consecutive blood meals on female BALB/c mice treated with NeemAzal at an azadirachtin A concentration of 60, 105 or 150 mg/kg. The blood feeding capacity was estimated by measuring the haematin content of the rectal fluid excreted by the mosquitoes during feeding. The number of eggs laid was estimated by image analysis and their hatchability assessed by direct observations. RESULTS: A dose and frequency dependent impact of NeemAzal treatment on the mosquito feeding capacity, oviposition and egg hatchability was demonstrated. In the 150 mg/kg treatment group, the mosquito feeding capacity was reduced by 50% already at the second blood meal and by 50 to 80% in all treatment groups at the fifth blood meal. Consequently, a 50 - 65% reduction in the number of eggs laid per female mosquito was observed after the fifth blood meal in all treatment groups. Similarly, after the fifth treated blood meal exposure, hatchability was found to be reduced by 62% and 70% in the 105 and 150 mg/kg group resp
AU  - Dembo,EG
AU  - Abay,SM
AU  - Dahiya,N
AU  - Ogboi,JS
AU  - Christophides,GK
AU  - Lupidi,G
AU  - Chianese,G
AU  - Lucantoni,L
AU  - Habluetzel,A
DO  - 10.1186/s13071-015-0700-1
PY  - 2015///
TI  - Impact of repeated NeemAzal-treated blood meals on the fitness of Anopheles stephensi mosquitoes.
T2  - Parasit Vectors
UR  - http://dx.doi.org/10.1186/s13071-015-0700-1
UR  - https://www.ncbi.nlm.nih.gov/pubmed/25884799
VL  - 8
ER  -
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