Imperial College London
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Professor Lesley Hoyles

Faculty of Medicine, Department of Surgery & Cancer

Visiting Professor
 
 
 
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Contact

 

lesley.hoyles11 Website

 
 
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Location

 

Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hoyles:2015:10.7717/peerj.1061,
author = {Hoyles, L and Murphy, J and Neve, H and Heller, KJ and Turton, JF and Mahony, J and Sanderson, JD and Hudspith, B and Gibson, GR and McCartney, AL and van, Sinderen D},
doi = {10.7717/peerj.1061},
journal = {PeerJ},
title = {Klebsiella pneumoniae subsp. pneumoniae – bacteriophagecombination from the caecal effluent of a healthy woman},
url = {http://dx.doi.org/10.7717/peerj.1061},
volume = {3},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A sample of caecal effluent was obtained from a female patient who had undergone a routine colonoscopic examination. Bacteria were isolated anaerobically from the sample, and screened against the remaining filtered caecal effluent in an attempt to isolate bacteriophages (phages). A lytic phage, named KLPN1, was isolated on a strain identified as Klebsiella pneumoniae subsp. pneumoniae (capsular type K2, rmpA+). This Siphoviridae phage presents a rosette-like tail tip and exhibits depolymerase activity, as demonstrated by the formation of plaque-surrounding haloes that increased in size over the course of incubation. When screened against a panel of clinical isolates of K. pneumoniae subsp. pneumoniae, phage KLPN1 was shown to infect and lyse capsular type K2 strains, though it did not exhibit depolymerase activity on such hosts. The genome of KLPN1 was determined to be 49,037 bp (50.53 %GC) in length, encompassing 73 predicted ORFs, of which 23 represented genes associated with structure, host recognition, packaging, DNA replication and cell lysis. On the basis of sequence analyses, phages KLPN1 (GenBank: KR262148) and 1513 (a member of the family Siphoviridae, GenBank: KP658157) were found to be two new members of the genus “Kp36likevirus.”
AU  - Hoyles,L
AU  - Murphy,J
AU  - Neve,H
AU  - Heller,KJ
AU  - Turton,JF
AU  - Mahony,J
AU  - Sanderson,JD
AU  - Hudspith,B
AU  - Gibson,GR
AU  - McCartney,AL
AU  - van,Sinderen D
DO  - 10.7717/peerj.1061
PY  - 2015///
SN  - 2167-8359
TI  - Klebsiella pneumoniae subsp. pneumoniae – bacteriophagecombination from the caecal effluent of a healthy woman
T2  - PeerJ
UR  - http://dx.doi.org/10.7717/peerj.1061
UR  - http://hdl.handle.net/10044/1/24981
VL  - 3
ER  -
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