Imperial College London
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Dr Olivier E. Pardo

Faculty of Medicine, Department of Surgery & Cancer

Reader in Cancer Cell Signalling
 
 
 
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Contact

 

+44 (0)20 7594 2814o.pardo Website CV

 
 
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Location

 

145ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lukacs:1996,
author = {Lukacs, KV and Pardo, O and Porter, C and Browning, J and Caplen, NJ and Manvell, M and Collins, MKL and Geddes, DM and Alton, EWFW},
journal = {Thorax},
title = {In vivo transfer of the β -galactosidase gene into malignant mesothelioma cells induces tumour rejection},
volume = {51},
year = {1996}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Malignant mesothelioma is an aggressive, fatal serosal tumour with no effective treatment. However, the potential accessibility of the tumour, localized to the pleural or peritoneal cavities, and the relative absence of metastases makes it a suitable candidate for gene therapy. In order to optimize the gene delivery system we used the β-galactosidase (β-gal) reporter gene delivered into a model of malignant mesothelioma. Mice were injected with 106 AC29 tumour cells (an asbestos-induced mesothelioma cell line) into the peritoneal cavity. Three days later the β-gal gene was delivered either by retrovirus, non-capsulated retrovirus or cationic liposome-complexed plasmid DNA. Mice were sacrificed 12 days later, tumour size determined and both histological and quantitative β-gal assays carried out to detect transduced cells in tumour samples, spleen, liver and peritoneum. Untreated controls (tumor size: 1.68±0.15g) or animals treated with the vehicle (liposome) only (1.61±0.11g), rapidly developed disseminated tumours in the peritoneum. Mice injected with the non-capsulated virus showed no protection against tumour growth (1.88±0.17g). However, mice injected with retrovirus (0.65±0.1g) or plasmid-liposome complexes (0.14±0.12g) showed a highly significant decrease (p<0.001) in the tumour size. These data are in keeping with in vitro studies in which AC29 cells can be transfected with both retroviral and plasmid liposome vectors but not with the non-capsulated virus. However, neither histological analysis nor a quantitative assay detected β-gal positive cells in the tumour samples of treated mice. These results indicate the elimination of both transduced and untransduced tumour cells after in vivo gene transfer of a foreign protein. They also suggest that the efficiency of in vivo gene transfer is not accurately accessed by using bacterial marker genes, in keeping with data emerging in other areas of gene the
AU  - Lukacs,KV
AU  - Pardo,O
AU  - Porter,C
AU  - Browning,J
AU  - Caplen,NJ
AU  - Manvell,M
AU  - Collins,MKL
AU  - Geddes,DM
AU  - Alton,EWFW
PY  - 1996///
SN  - 0040-6376
TI  - In vivo transfer of the β -galactosidase gene into malignant mesothelioma cells induces tumour rejection
T2  - Thorax
VL  - 51
ER  -
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