Imperial College London
Alternate

ProfessorGuyRutter

Faculty of Medicine, Department of Medicine

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 3340g.rutter Website

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rutter:1994:10.1016/0143-4160(94)90002-7,
author = {Rutter, GA and Theler, JM and Li, G and Wollheim, CB},
doi = {10.1016/0143-4160(94)90002-7},
journal = {Cell Calcium},
pages = {71--80},
title = {Ca<sup>2+</sup> stores in insulin-secreting cells: lack of effect of cADP ribose},
url = {http://dx.doi.org/10.1016/0143-4160(94)90002-7},
volume = {16},
year = {1994}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Ca2+ stores were examined in several insulin secreting cell types by measuring uptake and release of Ca2+ by permeabilised cells. In pancreatic islet cells or INS-1 cells, <20% of the ATP-dependent, thapsigargin-sensitive Ca2+ pool could be released by saturating concentrations of inositol (1,4,5)P3 (InsP3). InsP3 released > 60% of the thapsigargin-sensitive Ca2+ pool in RINm5F cells. The total Ca2+ content of the thapsigargin-sensitive pool was similar in each of these cell types. Neither cADP ribose (cADPR; 1 μM) nor caffeine (10 mM) caused significant Ca2+ release from any of the permeabilised insulin-secreting cell preparations. ATP elicited similar increases in intracellular Ca2+ concentration ([Ca2+]i) in single, living INS-1 and RINm5F cells, and similar fold increases in InsP3 levels in cell populations. The Ca2+ ATPase inhibitor thapsigargin, added after ATP, caused smaller [Ca2+]i increases in RINm5F than in INS-1 cells. This is consistent with the presence of a smaller InsP3-sensitive Ca2+ pool in living INS-1 cells. The data indicate that InsP3 receptors are present in only a small subfraction of the Ca2+ ATPase-containing Ca2+ stores in INS-1 and pancreatic β-cells, and that cADP ribose/caffeine-sensitive Ca2+-induced Ca2+ release channels may be entirely absent from this endocrine cell type,. © 1994.
AU  - Rutter,GA
AU  - Theler,JM
AU  - Li,G
AU  - Wollheim,CB
DO  - 10.1016/0143-4160(94)90002-7
EP  - 80
PY  - 1994///
SN  - 0143-4160
SP  - 71
TI  - Ca<sup>2+</sup> stores in insulin-secreting cells: lack of effect of cADP ribose
T2  - Cell Calcium
UR  - http://dx.doi.org/10.1016/0143-4160(94)90002-7
VL  - 16
ER  -
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