Oblique plane microscopy (OPM) [1] is a light sheet microscopy approach that utilises a single microscope objective lens for both excitation and imaging and which can be implemented on a standard microscope frame – making it compatible with most existing sample formats including samples arrayed in multiwell plates. OPM utilises remote focussing to image a tilted plane in the specimen this also facilitates convenient rapid scanning of the light sheet to provide fast (up to 25 vol/s) volumetric imaging for time-resolved 3D studies, e.g. of dynamic or spontaneous processes such as calcium sparks in beating cardiomyocytes [2] and high throughput 3D imaging of multiwell plate arrays of samples such as 3D cell cultures for rapid (time-lapse) 3D high content analysis [3].

OPM schematic

Details of specific OPM instrument configurations can be found in the following references:

  1. Optically sectioned imaging by oblique plane microscopy 
    C. Dunsby, Optics Express 16 (2008) 20306-20316

  2. High-speed 2D and 3D fluorescence microscopy of cardiac myocytes 
    Sunil Kumar, Dean Wilding, Markus B. Sikkel, Alexander R. Lyon, Ken T. MacLeod, and Chris Dunsby, Opt. Express 19(2011) 13839-13847

  3. Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates
    Vincent Maioli, George Chennell, Hugh Sparks, Tobia Lana, Sunil Kumar, David Carling, Alessandro Sardini & Chris Dunsby, Sci Rep 6, (2016) 37777