openFrame: an open source approach to fluorescence microscopy

We are developing an open microscopy hardware platform that aims to enable modular, upgradeable and easily maintained microscope systems to be assembled rapidly using a core modular microscope frame with add-on modules such as excitation couplers or illuminators and camera systems that can be implemented using widely available commercial components and systems. We intend this microscopy platform to be controlled using MicroManager and we will share our software as we develop it. 

We will also share the CAD designs and component lists so that other researchers can easily replicate the instrumentation or adapt it to their requirements. 

To download a 3D PDF of openFrame, please download this file and view it in Acrobat, as this may not display properly in a browser: openFrame animation PDF

The most recent CAD files for the openFrame modules can be downloaded here: openFrame CAD files 20200130‌

This computer-aided design was developed by Simon Johnson and Frederik Goerlitz at Imperial College London and Callum Hollick at Cairn Research Ltd. The openFrame instrument was also designed and evaluated by Sunil Kumar.  Chris Dunsby, Martin Kehoe, Mark Neil and Paul French from Imperial and Jeremy Graham from Cairn Research Ltd also contributed to the design of openFrame. 

If you would like to build in additional functionality or to know about components that have been tested with the openFrame, please see this parts list and schematic (XLSX) for a TIRF illuminator, a camera port and an excitation source, which also lists an objective lens and focus control equipment that have been tested on the system.