In this section
@article{Zeden:2023:10.1101/pdb.prot107948,
author = {Zeden, MS and Schuster, CF and Gründling, A},
doi = {10.1101/pdb.prot107948},
journal = {Cold Spring Harb Protoc},
title = {Allelic-Exchange Procedure in Staphylococcus aureus.},
url = {http://dx.doi.org/10.1101/pdb.prot107948},
volume = {2023},
year = {2023}
}
TY - JOUR
AB - This protocol continues a series of methods for the construction of an in-frame gene deletion in Staphylococcus aureus strain RN4220. To this end, we describe in this protocol an allelic-exchange procedure for S. aureus We have previously described how an allelic-exchange plasmid containing a desired gene deletion (in this case, pIMAY-ΔtagO) can be constructed and isolated from Escherichia coli, then introduced into electrocompetent S. aureus cells by electroporation. This plasmid contains a temperature-sensitive origin of replication, a counterselectable marker (pheS gene) and confers chloramphenicol resistance to S. aureus As a specific example, we present the construction of strain RN4220ΔtagO from strain RN4220 carrying the pIMAY-ΔtagO plasmid. The protocol can be easily adapted for the construction of other gene deletions and/or allelic-exchange plasmids.
AU - Zeden,MS
AU - Schuster,CF
AU - Gründling,A
DO - 10.1101/pdb.prot107948
PY - 2023///
TI - Allelic-Exchange Procedure in Staphylococcus aureus.
T2 - Cold Spring Harb Protoc
UR - http://dx.doi.org/10.1101/pdb.prot107948
UR - https://www.ncbi.nlm.nih.gov/pubmed/37117017
VL - 2023
ER -