In this section
@article{Zeden:2023:10.1101/pdb.top107945,
author = {Zeden, MS and Schuster, CF and Gründling, A},
doi = {10.1101/pdb.top107945},
journal = {Cold Spring Harb Protoc},
title = {Allelic Exchange: Construction of an Unmarked In-Frame Deletion in Staphylococcus aureus.},
url = {http://dx.doi.org/10.1101/pdb.top107945},
volume = {2023},
year = {2023}
}
TY - JOUR
AB - Here we describe an allelic-exchange procedure for the construction of an unmarked gene deletion in the bacterium Staphylococcus aureus As a practical example, we outline the construction of a tagO gene deletion in S. aureus using the allelic-exchange plasmid pIMAY. We first present the general principles of the allelic-exchange method, along with information on counterselectable markers. Furthermore, we summarize relevant cloning procedures, such as the splicing by overhang extension (SOE) polymerase chain reaction (PCR) and Gibson assembly methods, and we conclude by giving some general consideration to performing genetic modifications in S. aureus.
AU - Zeden,MS
AU - Schuster,CF
AU - Gründling,A
DO - 10.1101/pdb.top107945
PY - 2023///
TI - Allelic Exchange: Construction of an Unmarked In-Frame Deletion in Staphylococcus aureus.
T2 - Cold Spring Harb Protoc
UR - http://dx.doi.org/10.1101/pdb.top107945
UR - https://www.ncbi.nlm.nih.gov/pubmed/37117029
VL - 2023
ER -