BibTex format
@article{Fadini:2023:10.1021/jacs.3c02313,
author = {Fadini, A and Hutchison, CDM and Morozov, D and Chang, J and Maghlaoui, K and Perrett, S and Luo, F and Kho, JCX and Romei, MG and Morgan, RML and Orr, CM and Cordon-Preciado, V and Fujiwara, T and Nuemket, N and Tosha, T and Tanaka, R and Owada, S and Tono, K and Iwata, S and Boxer, SG and Groenhof, G and Nango, E and van, Thor JJ},
doi = {10.1021/jacs.3c02313},
journal = {Journal of the American Chemical Society},
pages = {15796--15808},
title = {Serial femtosecond crystallography reveals that photoactivation in a fluorescent protein proceeds via the hula twist mechanism},
url = {http://dx.doi.org/10.1021/jacs.3c02313},
volume = {145},
year = {2023}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Chromophore cis/trans photoisomerization is a fundamental process in chemistry and in the activation of many photosensitive proteins. A major task is understanding the effect of the protein environment on the efficiency and direction of this reaction compared to what is observed in the gas and solution phases. In this study, we set out to visualize the hula twist (HT) mechanism in a fluorescent protein, which is hypothesized to be the preferred mechanism in a spatially constrained binding pocket. We use a chlorine substituent to break the twofold symmetry of the embedded phenolic group of the chromophore and unambiguously identify the HT primary photoproduct. Through serial femtosecond crystallography, we then track the photoreaction from femtoseconds to the microsecond regime. We observe signals for the photoisomerization of the chromophore as early as 300 fs, obtaining the first experimental structural evidence of the HT mechanism in a protein on its femtosecond-to-picosecond timescale. We are then able to follow how chromophore isomerization and twisting lead to secondary structure rearrangements of the protein β-barrel across the time window of our measurements.
AU - Fadini,A
AU - Hutchison,CDM
AU - Morozov,D
AU - Chang,J
AU - Maghlaoui,K
AU - Perrett,S
AU - Luo,F
AU - Kho,JCX
AU - Romei,MG
AU - Morgan,RML
AU - Orr,CM
AU - Cordon-Preciado,V
AU - Fujiwara,T
AU - Nuemket,N
AU - Tosha,T
AU - Tanaka,R
AU - Owada,S
AU - Tono,K
AU - Iwata,S
AU - Boxer,SG
AU - Groenhof,G
AU - Nango,E
AU - van,Thor JJ
DO - 10.1021/jacs.3c02313
EP - 15808
PY - 2023///
SN - 0002-7863
SP - 15796
TI - Serial femtosecond crystallography reveals that photoactivation in a fluorescent protein proceeds via the hula twist mechanism
T2 - Journal of the American Chemical Society
UR - http://dx.doi.org/10.1021/jacs.3c02313
UR - https://pubs.acs.org/doi/10.1021/jacs.3c02313
VL - 145
ER -