In this section
@article{Scheid:1998,
author = {Scheid, P and Griesenbach, U and Davies, J and Siddique, Z and Gruenert, DC and Geddes, DM and Alton, EWFW},
journal = {Thorax},
title = {Interleukin-8 production by airway epithelium and the effect of liposome-mediated IκB gene transfer},
volume = {53},
year = {1998}
}
TY - JOUR
AB - Interleukin-8 (IL-8) is a major chemoattractant for neutrophils, present in the CF lung in large numbers. We measured basal and stimulated IL-8 secretion from CF and non CF airway epithelial cell lines (CFTE and 16HBE cells, respectively) as well as from Cos7 cells in a controlled in vitro environment. Basal IL-8 secretion was significantly higher in CFTE cells (8110 ± 2286 pg/mg protein) than in 16HBE (1113 ± 123 pg/mg protein) and Cos7 (497 ± 138 pg/mg protein) [n=6, p<0.05]. Following TNF-α stimulation IL-8 secretion increased 8 ± 0.9 fold in CFTE, 2 ± 0.4 fold in 16HBE and 2.7 ± 0.2 fold in Cos7 cells. Preliminary data in freshly obtained human nasal cells also indicate an increased basal secretion of IL-8 (non CF: 62 ± 10 pg/ml n=16, CF: 165 ± 37 pg/ml n=12, p<0.05). The response to addition of Pseudomonas aeruginosa to these cells as measured by IL-8 secretion was also increased in CF (3279 ± 656 pg/ml n=10, p<0.05) when compared to non CF samples (817 ± 143 pg/ml n=8). Thus it appears that the IL-8 response is disproportionately greater in CF cells. It is likely that new treatment aimed at reducing IL-8 levels will be beneficial in CF. Nuclear factor kappa B (NFκB) is a transcription factor that regulates expression of inflammatory mediators including IL-8. NFκB is kept in an inactive state by association with a specific inhibitor (IκB). We investigated the effects of liposome-mediated overexpression of IκB in CFTE and Cos7 cells. Despite successful transfection, as confirmed by RT-PCR in both cell types, we did not detect an alteration of basal or TNF-α stimulated IL-8 secretion. This is most likely related to low levels of gene expression. In conclusion, we have shown that CF cells lines as well as CF primary cells produce increased levels of secreted IL-8 in the basal as well as in a stimulated state. Reduction of IL-8 secretion is likely to b
AU - Scheid,P
AU - Griesenbach,U
AU - Davies,J
AU - Siddique,Z
AU - Gruenert,DC
AU - Geddes,DM
AU - Alton,EWFW
PY - 1998///
SN - 0040-6376
TI - Interleukin-8 production by airway epithelium and the effect of liposome-mediated IκB gene transfer
T2 - Thorax
VL - 53
ER -
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