In this section
@article{Davies:1996,
author = {Davies, J and Dewar, A and Bush, A and Geddes, DM and Alton, EWFW},
journal = {Thorax},
title = {Pseudomonas aeruginosa adherence to cystic fibrosis respiratory epithelium is reduced by anti-asialo GM1 antibody and neuraminidase inhibition},
volume = {51},
year = {1996}
}
TY - JOUR
AB - Pseudomonas aeruginosa (P. aer), the bacterial pathogen responsible for most of the lung damage in cystic fibrosis (CF), adheres in greater numbers to cells of CF origin than to non-CF cells, which in part may explain the close relationship between this infection and CF. We have previously shown that this increased adherence results from the absence of normal CFTR, in that P. aer binding is reduced after in vitro liposome-mediated CFTR gene transfer. Previous studies have demonstrated that P. aer binds to asialylated glycolipids which are present in increased numbers on the surface of CF cells, and that neuraminidase, an exoproduct of P. aer, by cleaving sialic acid from glycolipids, can increase the availability of such binding sites. Mechanisms to reduce the adherence of P. aer to CF cells, thought to be the initial step in the process of infection, could potentially delay or prevent pulmonary colonisation and the deterioration in lung function which ensues. We have studied the possibility of reducing binding to asialo GM1 receptors, both by blocking with anti-asialo GM1 antibody, and with the inhibitor, 2,3-dehydo-2-deoxy-N-acetylneuraminic acid (DANA), to prevent neuraminidase-mediated increase in asialylated glycolipids. Nasal epithelial cells from 9 CF subjects were divided into 3 aliquots: each was exposed to a fixed concentration of a non-mucoid laboratory strain of P. aer (strain K), one aliquot after 1 hour incubation with a polyclonal rabbit anti-asialo GM1 antibody, and another in the presence of 50 μM DANA. A further 3 samples were used to study the affect of a control polyclonal anti-mouse antibody. Bacterial adherence to the apical surface of ciliated epithelial cells was quantified in a blinded fashion by direct visualisation using scanning electron microscopy. Pre-treatment with anti-asialo GM1 antibody resulted in a reduction in bacterial binding to every sample, with a mean reduction of 50% (mean binding /10 cells: baseline 9.7; anti-asialo GM1
AU - Davies,J
AU - Dewar,A
AU - Bush,A
AU - Geddes,DM
AU - Alton,EWFW
PY - 1996///
SN - 0040-6376
TI - Pseudomonas aeruginosa adherence to cystic fibrosis respiratory epithelium is reduced by anti-asialo GM1 antibody and neuraminidase inhibition
T2 - Thorax
VL - 51
ER -
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