BibTex format
@article{Ibrahim:2026:10.1126/science.aeb6690,
author = {Ibrahim, T and King, FJ and Toghani, A and Wang, L and Jenkins, S and Yuen, ELH and Wang, H-Y and Vuolo, C and Eilmann, N and Adamkova, V and Chia, K-S and Castel, B and Jones, JDG and Carella, P and Wu, C-H and Kourelis, J and Kamoun, S and Bozkurt, TO},
doi = {10.1126/science.aeb6690},
journal = {Science},
pages = {499--505},
title = {A helper NLR channels organellar calcium to trigger plant immunity.},
url = {http://dx.doi.org/10.1126/science.aeb6690},
volume = {392},
year = {2026}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Upon activation, plant nucleotide-binding leucine-rich repeat (NLR) immune receptors are known to assemble into oligomeric resistosomes that insert into the plasma membrane, forming calcium (Ca2+)-permeable channels and triggering immunity. Here, we found that the RPW8-like coiled-coil NLR (CCR-NLR) N requirement gene 1 (NRG1) primarily targets organelles instead of the plasma membrane. Unlike canonical CC-NLRs, activated NRG1 accumulated at the chloroplast envelope and channeled stromal Ca2+ into the cytosol. AlphaFold modeling of the NRG1 resistosome revealed an unusually long amino-terminal membrane-insertion structure that could span the double membrane of the chloroplast. Nanobody-mediated relocalization showed functional membrane specificity: Chloroplast trapping abolished activity of the canonical helper CC-NLR NRC4 but not NRG1. NRG1 orthologs, from nonflowering lineages to angiosperms, targeted chloroplasts, suggesting that organelle-centered defense dates back at least ~360 million years. We propose that CC-NLR diversification has enabled compartment-specific immune signaling to capture diverse Ca2+ stores.
AU - Ibrahim,T
AU - King,FJ
AU - Toghani,A
AU - Wang,L
AU - Jenkins,S
AU - Yuen,ELH
AU - Wang,H-Y
AU - Vuolo,C
AU - Eilmann,N
AU - Adamkova,V
AU - Chia,K-S
AU - Castel,B
AU - Jones,JDG
AU - Carella,P
AU - Wu,C-H
AU - Kourelis,J
AU - Kamoun,S
AU - Bozkurt,TO
DO - 10.1126/science.aeb6690
EP - 505
PY - 2026///
SP - 499
TI - A helper NLR channels organellar calcium to trigger plant immunity.
T2 - Science
UR - http://dx.doi.org/10.1126/science.aeb6690
UR - https://www.ncbi.nlm.nih.gov/pubmed/42060733
VL - 392
ER -