In this section
@article{Singh:2012:10.1007/978-1-61779-770-5_7,
author = {Singh, S and Gras, A and Fiez-Vandal, C and Martinez, M and Wagner, R and Byrne, B},
doi = {10.1007/978-1-61779-770-5_7},
journal = {Methods Mol Biol},
pages = {65--73},
title = {Screening for high-yielding Pichia pastoris clones: the production of G protein-coupled receptors as a case study.},
url = {http://dx.doi.org/10.1007/978-1-61779-770-5_7},
volume = {866},
year = {2012}
}
TY - JOUR
AB - Pichia pastoris is an established host for the production of a wide range of recombinant proteins including membrane proteins. The system has a particularly good track record for the production of G protein-coupled receptors (GPCRs). Generation and screening of expression clones with this system use standard molecular biology techniques. Multiple clones can be generated and screened in a matter of a few weeks making this similar to Escherichia coli in terms of speed. In addition, basic buffer components and the lack of expensive equipment make small-scale expression screening in P. pastoris very cost-effective. Here we describe the procedures used for small-scale GPCR production screening.
AU - Singh,S
AU - Gras,A
AU - Fiez-Vandal,C
AU - Martinez,M
AU - Wagner,R
AU - Byrne,B
DO - 10.1007/978-1-61779-770-5_7
EP - 73
PY - 2012///
SP - 65
TI - Screening for high-yielding Pichia pastoris clones: the production of G protein-coupled receptors as a case study.
T2 - Methods Mol Biol
UR - http://dx.doi.org/10.1007/978-1-61779-770-5_7
UR - https://www.ncbi.nlm.nih.gov/pubmed/22454115
VL - 866
ER -
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