In this section
@article{Singh:2012:10.1007/978-1-61779-770-5_17,
author = {Singh, S and Gras, A and Fiez-Vandal, C and Martinez, M and Wagner, R and Byrne, B},
doi = {10.1007/978-1-61779-770-5_17},
journal = {Methods Mol Biol},
pages = {197--207},
title = {Large-scale production of membrane proteins in Pichia pastoris: the production of G protein-coupled receptors as a case study.},
url = {http://dx.doi.org/10.1007/978-1-61779-770-5_17},
volume = {866},
year = {2012}
}
TY - JOUR
AB - One of the major advantages of using Pichia pastoris is that it is readily adapted to large-scale culture in bioreactors. Bioreactors allow precise regulation of cell growth parameters increasing both yields and reproducibility of the culture. P. pastoris cultures grow to very high cell densities which helps minimise culture volume and facilitates downstream processing of the sample. Here, we provide protocols for the large-scale production of the human adenosine A(2A) receptor (A(2A)R) and provide some details of how bioreactor cultures can be used for optimisation of expression of the human dopamine D2 receptor (D2DR).
AU - Singh,S
AU - Gras,A
AU - Fiez-Vandal,C
AU - Martinez,M
AU - Wagner,R
AU - Byrne,B
DO - 10.1007/978-1-61779-770-5_17
EP - 207
PY - 2012///
SN - 1064-3745
SP - 197
TI - Large-scale production of membrane proteins in Pichia pastoris: the production of G protein-coupled receptors as a case study.
T2 - Methods Mol Biol
UR - http://dx.doi.org/10.1007/978-1-61779-770-5_17
VL - 866
ER -
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